Impaired plasma cholesteryl ester transfer with accumulation of larger high density lipoproteins in some families of baboons (Papio sp.)

R. S. Kushwaha, D. L. Rainwater, M. C. Williams, G. S. Getz, H. C. McGill

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Baboons from some families have a higher concentration of plasma high density lipoproteins (HDL) on a chow diet and accumulate large HDL (HDL1) when challenged with a high cholesterol and high saturated fat (HCHF) diet. HDL1 from high HDL1 animals contained more (1.5-fold) cholesteryl ester than HDL (HDL2 + HDL3) from high or low HDL1 animals. HDL from high HDL1 baboons had lower triglyceride content than that from low HDL1 baboons. HDL3 or HDL labeled with [3H]cholesteryl linoleate was incubated with entire lipoprotein fraction (d < 1.21 g/ml) or very low density lipoprotein + low density lipoprotein (VLDL + LDL) (d < 1.045 g/ml) and with lipoprotein-deficient serum (LPDS), and the radioactive cholesteryl ester and mass floating at d 1.045 g/ml (VLDL + LDL) after the incubation was measured. The transfer of cholesteryl esters from either HDL or HDL3 prepared from plasma of high HDL1 animals fed chow or the HCHF diet, was slower than the transfer from either HDL or HDL3 of low HDL1 animals, regardless of the source of transfer activity or the ratio of LDL:HDL-protein used in the assay. Addition of HDL from high HDL1 baboons into an assay mixture of plasma components from low HDL1 baboons decreased the transfer of cholesteryl ester radioactivity and mass from HDL to VLDL and LDL. In addition to HDL, a fraction of intermediate density lipoprotein (IDL) and denser HDL were also effective in inhibiting the transfer. These observations suggest that accumulation of HDL1 in high HDL1 baboons fed an HCHF diet is associated with a slower transfer of cholesteryl esters from HDL to LDL. This slower transfer of cholesteryl esters appears to be related to HDL protein composition rather than differences in transfer protein activity.

Original languageEnglish (US)
Pages (from-to)965-973
Number of pages9
JournalJournal of Lipid Research
Volume31
Issue number6
StatePublished - 1990
Externally publishedYes

Fingerprint

Papio
Cholesterol Esters
HDL Lipoproteins
Plasmas
HDL3 Lipoprotein
High Fat Diet
Nutrition
Animals
VLDL Lipoproteins
Cholesterol
LDL Lipoproteins
Lipoproteins
Fats
HDL2 Lipoprotein
IDL Lipoproteins
Assays
Proteins
Radioactivity
Triglycerides
Diet

Keywords

  • apoA-I
  • cholesteryl ester transfer protein
  • dietary cholesterol and fat
  • hyperalphalipoproteinemia
  • hyperlipidemia
  • low density lipoproteins

ASJC Scopus subject areas

  • Endocrinology

Cite this

Kushwaha, R. S., Rainwater, D. L., Williams, M. C., Getz, G. S., & McGill, H. C. (1990). Impaired plasma cholesteryl ester transfer with accumulation of larger high density lipoproteins in some families of baboons (Papio sp.). Journal of Lipid Research, 31(6), 965-973.

Impaired plasma cholesteryl ester transfer with accumulation of larger high density lipoproteins in some families of baboons (Papio sp.). / Kushwaha, R. S.; Rainwater, D. L.; Williams, M. C.; Getz, G. S.; McGill, H. C.

In: Journal of Lipid Research, Vol. 31, No. 6, 1990, p. 965-973.

Research output: Contribution to journalArticle

Kushwaha, R. S. ; Rainwater, D. L. ; Williams, M. C. ; Getz, G. S. ; McGill, H. C. / Impaired plasma cholesteryl ester transfer with accumulation of larger high density lipoproteins in some families of baboons (Papio sp.). In: Journal of Lipid Research. 1990 ; Vol. 31, No. 6. pp. 965-973.
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