Impaired development of early endometriotic lesions in CD44 knockout mice

Jennifer F. Knudtson, Rajeshwar R. Tekmal, Marlen Tellez Santos, Peter A. Binkley, Naveen Krishnegowda, Philip Valente, Robert S. Schenken

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Previous studies have shown endometrial cell (EC) CD44 and peritoneal mesothelial cell (PMC)-associated hyaluronan (hyaluronic acid [HA]) are involved in the attachment of endometrial stroma and epithelial cells to peritoneal mesothelium. Here we assess the CD44-HA interaction in the formation of the early endometriotic lesion using CD44-/- (knockout) mice. Using an established murine model and crossover technique, endometrial tissue from donor mice (wild type [WT] and CD44-/-) was used to induce endometriosis in recipient mice (WT and CD44-/-). Endometriotic lesions were visualized by fluorescent microscopy and confirmed by hematoxylin and eosin staining. Early endometriotic lesions were decreased when CD44-/- endometrium was placed in WT recipients and when WT endometrium was placed in CD44-/- recipients (P = .002). Early endometriotic lesions were also significantly decreased when both peritoneal and endometrial tissues lacked CD44 expression (P < .01). These studies demonstrate that both EC and PMC CD44 play a role in the development of early endometriotic lesion.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalReproductive Sciences
Volume23
Issue number1
DOIs
StatePublished - Jan 2016

Keywords

  • CD44
  • Endometriosis
  • Endometriosis model
  • Hyaluronic acid

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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