Abstract
Previous studies have shown endometrial cell (EC) CD44 and peritoneal mesothelial cell (PMC)-associated hyaluronan (hyaluronic acid [HA]) are involved in the attachment of endometrial stroma and epithelial cells to peritoneal mesothelium. Here we assess the CD44-HA interaction in the formation of the early endometriotic lesion using CD44-/- (knockout) mice. Using an established murine model and crossover technique, endometrial tissue from donor mice (wild type [WT] and CD44-/-) was used to induce endometriosis in recipient mice (WT and CD44-/-). Endometriotic lesions were visualized by fluorescent microscopy and confirmed by hematoxylin and eosin staining. Early endometriotic lesions were decreased when CD44-/- endometrium was placed in WT recipients and when WT endometrium was placed in CD44-/- recipients (P = .002). Early endometriotic lesions were also significantly decreased when both peritoneal and endometrial tissues lacked CD44 expression (P < .01). These studies demonstrate that both EC and PMC CD44 play a role in the development of early endometriotic lesion.
Original language | English (US) |
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Pages (from-to) | 87-91 |
Number of pages | 5 |
Journal | Reproductive Sciences |
Volume | 23 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2016 |
Keywords
- CD44
- Endometriosis
- Endometriosis model
- Hyaluronic acid
ASJC Scopus subject areas
- Obstetrics and Gynecology