Immunosuppressive properties of surfactant in alveolar macrophage NR8383

J. Kerecman, S. B. Mustafa, M. M. Vasquez, P. S. Dixon, R. Castro

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Objective: To evaluate the anti-inflammatory effects of exogenous surfactants and surfactant phospholipid without surfactant proteins (SP-A and SP-D) on the lipopolysaccharide- (LPS) stimulated rat alveolar macrophage (AM) cell line NR8383. Methods: Exogenous surfactants (beractant, calfactant or colfosceril) and surfactant phospholipid (dipalmitoyl phosphatidylcholine, DPPC), standardized to phospholipid content of 25-1,000 μg/ml were incubated with LPS- (1 μg/ml) stimulated NR8383 AMs. Results: TNF-α and IL-1β secretion and nitric oxide (NO) formation following LPS stimulation were inhibited by treatment with surfactants or DPPC. Furthermore, LPS-dependent NO production and iNOS protein levels were significantly suppressed in cells pretreated for one hour with beractant compared to beractant added simultaneously with or following LPS. Additionally, LPS-stimulated oxidative burst, measured by flow cytometry, was significantly decreased by beractant. Finally, beractant inhibited the translocation of NF-κB from cytoplasmic into nuclear extract in LPS-stimulated NR8383 AMs. Conclusions: Exogenous surfactants and surfactant phospholipid inhibit secretion of proinflammatory cytokines and NO in NR8383 AMs. The inhibitory effects of beractant on oxygen radical and LPS-induced NO formation may result from unique mechanisms of decreasing cell signaling. The anti-inflammatory activity of surfactant products used in the treatment of neonatal respiratory distress syndrome (RDS) may depend upon the specific preparation or dose used.

Original languageEnglish (US)
Pages (from-to)118-125
Number of pages8
JournalInflammation Research
Volume57
Issue number3
DOIs
StatePublished - Mar 1 2008

Keywords

  • Alveolar macrophages
  • Cytokines
  • LPS
  • Nitric oxide (NO)
  • Surfactant

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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