Immunoreactive lectins in leaf gel from Aloe barbadensis Miller

Wendell D. Winters

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Lectins isolated from the gel portion of leaves of Aloe barbadensis Miller by differential centrifugations and gel filtration were found to have strong haemagglutination and mitogenic activities. Haemagglutination titres induced by these lectins were markedly higher than those induced by lectins isolated similarly from Aloe saponaria Haw and Aloe chinesis. The levels of lymphocyte blastogenesis using the Aloe barbadensis Miller lectins were high, but markedly less than those detected using positive control lectins concanavalin A and phytohae‐magglutinin. Results of Western blot transfers followed by immunoblots using specific receptor antisera suggested that the Aloe barbadensis Miller lectins were active at alpha D‐glucose and mannose sites and not at n‐acetyl glucosamine sites. The haemagglutination activities of these Aloe substances were also observed to decrease as a function of time frozen at liquid nitrogen temperatures for up to 9 years.

Original languageEnglish (US)
Pages (from-to)S23-S25
JournalPhytotherapy Research
Volume7
Issue number7
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • Aloe
  • lectins
  • liquid nitrogen stability
  • mitogens
  • sugar receptors

ASJC Scopus subject areas

  • Pharmacology

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