Immunolocalization of the inducible nitric oxide synthase isoform in human fetal membranes

A. L W Eis, D. E. Brockman, L. Myatt

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

PROBLEM: Nitric oxide (NO) synthesized by fetal membranes may protect the fetus from maternal infection or immune challenge or have a tocolytic effect on myometrium. The sites of synthesis and enzymes responsible for NO production in human fetal membranes remain unidentified. METHOD OF STUDY: Fetal membranes were obtained from four groups of patients: term (> 37 weeks gestation) or preterm (< 37 weeks gestation), both either in labor or not in labor. Frozen sections of membrane rolls were immunostained for inducible (iNOS) and endothelial (eNOS) nitric oxide synthase isoforms and the monocyte/macrophage marker CD14. RESULTS: Positive iNOS immunostaining was found in fibroblasts of amnionic and chorionic mesenchyme and in decidual macrophages identified by CD14 from all four groups of tissues. No iNOS immunostaining was seen in amnion epithelium or chorion trophoblast. Very intense iNOS staining was seen with evidence of monocyte/macrophage invasion of membranes. eNOS immunostaining was only found in decidual vascular endothelium. CONCLUSIONS: Constitutive expression of iNOS in decidual macrophages and fetal membrane fibroblasts may form an immune barrier against maternal insult. In chorioamnionitis, macrophage recruitment and NO expression may be part of the maternal immune response.

Original languageEnglish (US)
Pages (from-to)289-294
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume38
Issue number4
StatePublished - 1997
Externally publishedYes

Fingerprint

Extraembryonic Membranes
Nitric Oxide Synthase Type II
Protein Isoforms
Macrophages
Nitric Oxide
Mothers
Monocytes
Fibroblasts
Tocolytic Agents
Chorioamnionitis
Chorion
Pregnancy
Amnion
Membranes
Myometrium
Nitric Oxide Synthase Type III
Vascular Endothelium
Trophoblasts
Frozen Sections
Mesoderm

Keywords

  • Chorioamnionitis
  • Fibroblast
  • Labor
  • Macrophage
  • Nitric oxide
  • Parturition

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Immunolocalization of the inducible nitric oxide synthase isoform in human fetal membranes. / Eis, A. L W; Brockman, D. E.; Myatt, L.

In: American Journal of Reproductive Immunology, Vol. 38, No. 4, 1997, p. 289-294.

Research output: Contribution to journalArticle

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AB - PROBLEM: Nitric oxide (NO) synthesized by fetal membranes may protect the fetus from maternal infection or immune challenge or have a tocolytic effect on myometrium. The sites of synthesis and enzymes responsible for NO production in human fetal membranes remain unidentified. METHOD OF STUDY: Fetal membranes were obtained from four groups of patients: term (> 37 weeks gestation) or preterm (< 37 weeks gestation), both either in labor or not in labor. Frozen sections of membrane rolls were immunostained for inducible (iNOS) and endothelial (eNOS) nitric oxide synthase isoforms and the monocyte/macrophage marker CD14. RESULTS: Positive iNOS immunostaining was found in fibroblasts of amnionic and chorionic mesenchyme and in decidual macrophages identified by CD14 from all four groups of tissues. No iNOS immunostaining was seen in amnion epithelium or chorion trophoblast. Very intense iNOS staining was seen with evidence of monocyte/macrophage invasion of membranes. eNOS immunostaining was only found in decidual vascular endothelium. CONCLUSIONS: Constitutive expression of iNOS in decidual macrophages and fetal membrane fibroblasts may form an immune barrier against maternal insult. In chorioamnionitis, macrophage recruitment and NO expression may be part of the maternal immune response.

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KW - Parturition

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