Immunolocalization of C1-tetrahydrofolate synthase in the rat kidney

Dean R. Appling; Suzanne H. Ayo; Jeffrey I. Kreisberg

doi:10.1016/0006-291X(90)92366-8

Immunolocalization of C₁-tetrahydrofolate synthase in the rat kidney

Dean R. Appling, Suzanne H. Ayo, Jeffrey I. Kreisberg

Research output: Contribution to journal › Article › peer-review

Abstract

The distribution of the trifunctional enzyme C₁-tetrahydrofolate synthase (C₁-THF synthase) was examined in the rat kidney by immunolocalization with anti-C₁-THF synthase serum using the peroxidase-antiperoxidase method. C₁-THF synthase immunoreactivity was detected in both distal and proximal epithelial cells. Staining of the distal tubule epithelia was more intense and granular whereas staining of the proximal tubule epithelia was diffuse. All cells of the cortical collecting duct showed positive granular staining. In the outer medullary collecting duct, the intercalated cells showed intense granular cytoplasmic staining and the principal cells were either negative or weakly positive. The ascending thick limb of Henle's loop was also positive. Glomeruli and the inner medulla showed no staining for C₁-THF synthase.

Original language	English (US)
Pages (from-to)	625-630
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	168
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(90)92366-8
State	Published - Apr 30 1990
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{8e7a3e4b158f45deb32b7f94b075de19,

title = "Immunolocalization of C1-tetrahydrofolate synthase in the rat kidney",

abstract = "The distribution of the trifunctional enzyme C1-tetrahydrofolate synthase (C1-THF synthase) was examined in the rat kidney by immunolocalization with anti-C1-THF synthase serum using the peroxidase-antiperoxidase method. C1-THF synthase immunoreactivity was detected in both distal and proximal epithelial cells. Staining of the distal tubule epithelia was more intense and granular whereas staining of the proximal tubule epithelia was diffuse. All cells of the cortical collecting duct showed positive granular staining. In the outer medullary collecting duct, the intercalated cells showed intense granular cytoplasmic staining and the principal cells were either negative or weakly positive. The ascending thick limb of Henle's loop was also positive. Glomeruli and the inner medulla showed no staining for C1-THF synthase.",

author = "Appling, {Dean R.} and Ayo, {Suzanne H.} and Kreisberg, {Jeffrey I.}",

note = "Funding Information: We would like to thank Barbara Goldschlag for preparation of the pre-absorbed antiserum. This work was supportedby GrantsDK36913 (D.R.A.) andDK29787 (J.1.K) from the National Institutes of Health, and a grant from the Foundation for Research.",

year = "1990",

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day = "30",

doi = "10.1016/0006-291X(90)92366-8",

language = "English (US)",

volume = "168",

pages = "625--630",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - Immunolocalization of C1-tetrahydrofolate synthase in the rat kidney

AU - Appling, Dean R.

AU - Ayo, Suzanne H.

AU - Kreisberg, Jeffrey I.

N1 - Funding Information: We would like to thank Barbara Goldschlag for preparation of the pre-absorbed antiserum. This work was supportedby GrantsDK36913 (D.R.A.) andDK29787 (J.1.K) from the National Institutes of Health, and a grant from the Foundation for Research.

PY - 1990/4/30

Y1 - 1990/4/30

N2 - The distribution of the trifunctional enzyme C1-tetrahydrofolate synthase (C1-THF synthase) was examined in the rat kidney by immunolocalization with anti-C1-THF synthase serum using the peroxidase-antiperoxidase method. C1-THF synthase immunoreactivity was detected in both distal and proximal epithelial cells. Staining of the distal tubule epithelia was more intense and granular whereas staining of the proximal tubule epithelia was diffuse. All cells of the cortical collecting duct showed positive granular staining. In the outer medullary collecting duct, the intercalated cells showed intense granular cytoplasmic staining and the principal cells were either negative or weakly positive. The ascending thick limb of Henle's loop was also positive. Glomeruli and the inner medulla showed no staining for C1-THF synthase.

AB - The distribution of the trifunctional enzyme C1-tetrahydrofolate synthase (C1-THF synthase) was examined in the rat kidney by immunolocalization with anti-C1-THF synthase serum using the peroxidase-antiperoxidase method. C1-THF synthase immunoreactivity was detected in both distal and proximal epithelial cells. Staining of the distal tubule epithelia was more intense and granular whereas staining of the proximal tubule epithelia was diffuse. All cells of the cortical collecting duct showed positive granular staining. In the outer medullary collecting duct, the intercalated cells showed intense granular cytoplasmic staining and the principal cells were either negative or weakly positive. The ascending thick limb of Henle's loop was also positive. Glomeruli and the inner medulla showed no staining for C1-THF synthase.

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U2 - 10.1016/0006-291X(90)92366-8

DO - 10.1016/0006-291X(90)92366-8

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AN - SCOPUS:0025195582

VL - 168

SP - 625

EP - 630

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

Immunolocalization of C₁-tetrahydrofolate synthase in the rat kidney

Abstract

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