Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas

W. A. Wesche, C. D.M. Fletcher, P. Dias, Peter J Houghton, D. M. Parham

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Adult pleomorphic rhabdomyosarcomas are difficult to distinguish from other pleomorphic soft tissue sarcomas. Immunostaining with the monoclonal antibody MyoD1 5.8A has recently shown promise as a sensitive and specific marker of rhabdomyosarcomas of childhood. To determine the usefulness of MyoD1 in distinguishing adult pleomorphic rhabdomyosarcoma from other pleomorphic sarcomas, we studied 21 cases of pleomorphic sarcoma, including six cases diagnosed as rhabdomyosarcoma. Immunostaining was accomplished using monoclonal antibodies against MyoD1, desmin, α-smooth-muscle actin, and muscle-specific actin on cryostat sections of frozen tumor. All cases of pleomorphic rhabdomyosarcoma stained positively with both desmin and MyoD1. Five cases diagnosed as nonrhabdomyosarcomatous pleomorphic sarcomas stained positively with desmin, but only one showed weak MyoD1 positivity. Three of these five cases were also positive with α-smooth-muscle actin. The results of this study suggest that the sensitivity and specificity of the MyoD1 antibody in the differential diagnosis of adult pleomorphic soft tissue sarcomas approaches that seen in pediatric rhabdomyosarcomas.

Original languageEnglish (US)
Pages (from-to)261-269
Number of pages9
JournalAmerican Journal of Surgical Pathology
Volume19
Issue number3
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Rhabdomyosarcoma
Sarcoma
Immunohistochemistry
Desmin
Actins
Smooth Muscle
Monoclonal Antibodies
Frozen Sections
Differential Diagnosis
Pediatrics
Sensitivity and Specificity
Muscles
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Anatomy
  • Surgery
  • Pathology and Forensic Medicine

Cite this

Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. / Wesche, W. A.; Fletcher, C. D.M.; Dias, P.; Houghton, Peter J; Parham, D. M.

In: American Journal of Surgical Pathology, Vol. 19, No. 3, 01.01.1995, p. 261-269.

Research output: Contribution to journalArticle

Wesche, W. A. ; Fletcher, C. D.M. ; Dias, P. ; Houghton, Peter J ; Parham, D. M. / Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. In: American Journal of Surgical Pathology. 1995 ; Vol. 19, No. 3. pp. 261-269.
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