Immunohistochemical study of 65-kDa oncofetal protein expression in breast cancer

M. Mirowski; J. Z. Blonski; H. Niewiadomska; B. Olborski; Z. Walaszek; M. Hanausek; L. Wozniak

doi:10.1016/S0960-9776(97)90006-0

Immunohistochemical study of 65-kDa oncofetal protein expression in breast cancer

M. Mirowski, J. Z. Blonski, H. Niewiadomska, B. Olborski, Z. Walaszek, M. Hanausek, L. Wozniak

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11 Scopus citations

Abstract

Western blot analysis of foetal bovine serum proteins (FBS) by monoclonal antibody produced against the 65-kDa tumour-associated phosphoprotein (p65) isolated from a human breast cancer cell line, showed the presence of an antigenically related (p65-like) protein in FBS. Four protein bands reacting with the anti-p65 monoclonal antibody were detected with an isoelectrofocusing gel (IEF). These four protein bands in SDS-PAGE exhibit the same molecular weight (about 65 kDa) but different isoelectric points (pIs) in IEF. A mixture of the purified isoforms of the p65-like protein was used for the immunization procedure. The usefulness of these anti-p65-like polyclonal antibodies for the immunohistochemical detection of p65 in breast cancer tissues was tested and compared with that of the anti-human-65 monoclonal antibodies. It can be concluded that the polyclonal antibodies obtained may be used in the detection of p65 antigen in breast cancer.

Original language	English (US)
Pages (from-to)	284-290
Number of pages	7
Journal	Breast
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/S0960-9776(97)90006-0
State	Published - Oct 1997
Externally published	Yes

ASJC Scopus subject areas

Surgery

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title = "Immunohistochemical study of 65-kDa oncofetal protein expression in breast cancer",

abstract = "Western blot analysis of foetal bovine serum proteins (FBS) by monoclonal antibody produced against the 65-kDa tumour-associated phosphoprotein (p65) isolated from a human breast cancer cell line, showed the presence of an antigenically related (p65-like) protein in FBS. Four protein bands reacting with the anti-p65 monoclonal antibody were detected with an isoelectrofocusing gel (IEF). These four protein bands in SDS-PAGE exhibit the same molecular weight (about 65 kDa) but different isoelectric points (pIs) in IEF. A mixture of the purified isoforms of the p65-like protein was used for the immunization procedure. The usefulness of these anti-p65-like polyclonal antibodies for the immunohistochemical detection of p65 in breast cancer tissues was tested and compared with that of the anti-human-65 monoclonal antibodies. It can be concluded that the polyclonal antibodies obtained may be used in the detection of p65 antigen in breast cancer.",

author = "M. Mirowski and Blonski, {J. Z.} and H. Niewiadomska and B. Olborski and Z. Walaszek and M. Hanausek and L. Wozniak",

year = "1997",

month = oct,

doi = "10.1016/S0960-9776(97)90006-0",

language = "English (US)",

volume = "6",

pages = "284--290",

journal = "Breast",

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TY - JOUR

T1 - Immunohistochemical study of 65-kDa oncofetal protein expression in breast cancer

AU - Mirowski, M.

AU - Blonski, J. Z.

AU - Niewiadomska, H.

AU - Olborski, B.

AU - Walaszek, Z.

AU - Hanausek, M.

AU - Wozniak, L.

PY - 1997/10

Y1 - 1997/10

N2 - Western blot analysis of foetal bovine serum proteins (FBS) by monoclonal antibody produced against the 65-kDa tumour-associated phosphoprotein (p65) isolated from a human breast cancer cell line, showed the presence of an antigenically related (p65-like) protein in FBS. Four protein bands reacting with the anti-p65 monoclonal antibody were detected with an isoelectrofocusing gel (IEF). These four protein bands in SDS-PAGE exhibit the same molecular weight (about 65 kDa) but different isoelectric points (pIs) in IEF. A mixture of the purified isoforms of the p65-like protein was used for the immunization procedure. The usefulness of these anti-p65-like polyclonal antibodies for the immunohistochemical detection of p65 in breast cancer tissues was tested and compared with that of the anti-human-65 monoclonal antibodies. It can be concluded that the polyclonal antibodies obtained may be used in the detection of p65 antigen in breast cancer.

AB - Western blot analysis of foetal bovine serum proteins (FBS) by monoclonal antibody produced against the 65-kDa tumour-associated phosphoprotein (p65) isolated from a human breast cancer cell line, showed the presence of an antigenically related (p65-like) protein in FBS. Four protein bands reacting with the anti-p65 monoclonal antibody were detected with an isoelectrofocusing gel (IEF). These four protein bands in SDS-PAGE exhibit the same molecular weight (about 65 kDa) but different isoelectric points (pIs) in IEF. A mixture of the purified isoforms of the p65-like protein was used for the immunization procedure. The usefulness of these anti-p65-like polyclonal antibodies for the immunohistochemical detection of p65 in breast cancer tissues was tested and compared with that of the anti-human-65 monoclonal antibodies. It can be concluded that the polyclonal antibodies obtained may be used in the detection of p65 antigen in breast cancer.

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EP - 290

JO - Breast

JF - Breast

SN - 0960-9776

IS - 5

ER -

Immunohistochemical study of 65-kDa oncofetal protein expression in breast cancer

Abstract

ASJC Scopus subject areas

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