Immunohistochemical localization of the NH2-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth

Guohua Yuan, Guobin Yang, Guangtai Song, Zhi Chen, Shuo Chen

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Dentin sialoprotein (DSP) is a major noncollagenous protein in dentin. Mutation studies in human, along with gene knockout and transgenic experiments in mice, have confirmed the critical role of DSP for dentin formation. Our previous study reported that DSP is processed into fragments in mouse odontoblast-like cells. In order to gain insights into the function of DSP fragments, we further evaluated the expression pattern of DSP in the mouse odontoblast-like cells using immunohistochemistry and western blot assay with antibodies against the NH2- terminal and COOH-terminal regions of DSP. Then, the distribution profiles of the DSP NH2-terminal and COOHterminal fragments and osteopontin (OPN) were investigated in mouse teeth at different ages by immunohistochemistry. In the odontoblast-like cells, multiple low molecular weight DSP fragments were detected, suggesting that part of the DSP protein was processed in the odontoblast-like cells. In mouse first lower molars, immunoreactions for anti-DSP-NH2 antibody were intense in the predentin matrix but weak in mineralized dentin; in contrast, for anti-DSPCOOH antibody, strong immunoreactions were found in mineralized dentin, in particular dentinal tubules but weak in predentin. Therefore, DSP NH2-terminal and COOHterminal fragments from odontoblasts were secreted to different parts of teeth, suggesting that they may play distinct roles in dentinogenesis. Meanwhile, both DSP antibodies showed weak staining in reactionary dentin (RD), whereas osteopontin (OPN) was clearly positive in RD. Therefore, DSP may be less crucial for RD formation than OPN.

Original languageEnglish (US)
Pages (from-to)605-614
Number of pages10
JournalCell and Tissue Research
Volume349
Issue number2
DOIs
StatePublished - Aug 2012

Keywords

  • Dentin
  • Dentin sialoprotein
  • Immunohistochemistry
  • Predentin
  • Reactionary dentin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Fingerprint Dive into the research topics of 'Immunohistochemical localization of the NH2-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth'. Together they form a unique fingerprint.

Cite this