TY - JOUR
T1 - Immunohistochemical localization of the NH2-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth
AU - Yuan, Guohua
AU - Yang, Guobin
AU - Song, Guangtai
AU - Chen, Zhi
AU - Chen, Shuo
N1 - Funding Information:
Acknowledgements This study was supported by grants from the National Natural Science Foundation of China (No. 81000436 and 81000418), the Fundamental Research Funds for the Central Universities (No. 4901049) and NIDCR (RO1 DE019892).
PY - 2012/8
Y1 - 2012/8
N2 - Dentin sialoprotein (DSP) is a major noncollagenous protein in dentin. Mutation studies in human, along with gene knockout and transgenic experiments in mice, have confirmed the critical role of DSP for dentin formation. Our previous study reported that DSP is processed into fragments in mouse odontoblast-like cells. In order to gain insights into the function of DSP fragments, we further evaluated the expression pattern of DSP in the mouse odontoblast-like cells using immunohistochemistry and western blot assay with antibodies against the NH2- terminal and COOH-terminal regions of DSP. Then, the distribution profiles of the DSP NH2-terminal and COOHterminal fragments and osteopontin (OPN) were investigated in mouse teeth at different ages by immunohistochemistry. In the odontoblast-like cells, multiple low molecular weight DSP fragments were detected, suggesting that part of the DSP protein was processed in the odontoblast-like cells. In mouse first lower molars, immunoreactions for anti-DSP-NH2 antibody were intense in the predentin matrix but weak in mineralized dentin; in contrast, for anti-DSPCOOH antibody, strong immunoreactions were found in mineralized dentin, in particular dentinal tubules but weak in predentin. Therefore, DSP NH2-terminal and COOHterminal fragments from odontoblasts were secreted to different parts of teeth, suggesting that they may play distinct roles in dentinogenesis. Meanwhile, both DSP antibodies showed weak staining in reactionary dentin (RD), whereas osteopontin (OPN) was clearly positive in RD. Therefore, DSP may be less crucial for RD formation than OPN.
AB - Dentin sialoprotein (DSP) is a major noncollagenous protein in dentin. Mutation studies in human, along with gene knockout and transgenic experiments in mice, have confirmed the critical role of DSP for dentin formation. Our previous study reported that DSP is processed into fragments in mouse odontoblast-like cells. In order to gain insights into the function of DSP fragments, we further evaluated the expression pattern of DSP in the mouse odontoblast-like cells using immunohistochemistry and western blot assay with antibodies against the NH2- terminal and COOH-terminal regions of DSP. Then, the distribution profiles of the DSP NH2-terminal and COOHterminal fragments and osteopontin (OPN) were investigated in mouse teeth at different ages by immunohistochemistry. In the odontoblast-like cells, multiple low molecular weight DSP fragments were detected, suggesting that part of the DSP protein was processed in the odontoblast-like cells. In mouse first lower molars, immunoreactions for anti-DSP-NH2 antibody were intense in the predentin matrix but weak in mineralized dentin; in contrast, for anti-DSPCOOH antibody, strong immunoreactions were found in mineralized dentin, in particular dentinal tubules but weak in predentin. Therefore, DSP NH2-terminal and COOHterminal fragments from odontoblasts were secreted to different parts of teeth, suggesting that they may play distinct roles in dentinogenesis. Meanwhile, both DSP antibodies showed weak staining in reactionary dentin (RD), whereas osteopontin (OPN) was clearly positive in RD. Therefore, DSP may be less crucial for RD formation than OPN.
KW - Dentin
KW - Dentin sialoprotein
KW - Immunohistochemistry
KW - Predentin
KW - Reactionary dentin
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U2 - 10.1007/s00441-012-1418-4
DO - 10.1007/s00441-012-1418-4
M3 - Article
C2 - 22581382
AN - SCOPUS:84871857230
SN - 0302-766X
VL - 349
SP - 605
EP - 614
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 2
ER -