Immunohistochemical localization of phosphoglycerate kinase isozymes in mouse testes

John L. Vandeberg, Chi‐Yu ‐Y Lee, Erwin Goldberg

    Research output: Contribution to journalArticlepeer-review

    16 Scopus citations

    Abstract

    We have used specific antibodies against mouse phosphoglycerate kinase (PGK) isozymes in an immunohistochemical technique to determine which cells in testicular cross sections contain PGK‐A and which contain PGK‐B. Phosphoglycerate kinase‐A is highly concentrated in interstitial cells and is weakly expressed in Sertoli cells and throughout the germinal epithelium. Phosphoglycerate kinase‐B is highly concentrated in spermatids and spermatozoa, but it is not detectable in stage 8 spermatids or earlier gametogenic stages, or in somatic cells of the testis. These results confirm that PGK‐B is a sperm‐specific isozyme in mice.

    Original languageEnglish (US)
    Pages (from-to)435-441
    Number of pages7
    JournalJournal of Experimental Zoology
    Volume217
    Issue number3
    DOIs
    StatePublished - Sep 1981

    ASJC Scopus subject areas

    • Animal Science and Zoology

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