Immunoglobulin class-switch DNA recombination: Induction, targeting and beyond

Zhenming Xu; Hong Zan; Egest J. Pone; Thach Mai; Paolo Casali

doi:10.1038/nri3216

Immunoglobulin class-switch DNA recombination: Induction, targeting and beyond

Zhenming Xu, Hong Zan, Egest J. Pone, Thach Mai, Paolo Casali

Research output: Contribution to journal › Review article › peer-review

293 Scopus citations

Abstract

Class-switch DNA recombination (CSR) of the immunoglobulin heavy chain (IGH) locus is central to the maturation of the antibody response and crucially requires the cytidine deaminase AID. CSR involves changes in the chromatin state and the transcriptional activation of the IGH locus at the upstream and downstream switch (S) regions that are to undergo S-S DNA recombination. In addition, CSR involves the induction of AID expression and the targeting of CSR factors to S regions by 14-3-3 adaptors, and it is facilitated by the transcription machinery and by histone modifications. In this Review, we focus on recent advances regarding the induction and targeting of CSR and outline an integrated model of the assembly of macromolecular complexes that transduce crucial epigenetic information to enzymatic effectors of the CSR machinery.

Original language	English (US)
Pages (from-to)	517-531
Number of pages	15
Journal	Nature Reviews Immunology
Volume	12
Issue number	7
DOIs	https://doi.org/10.1038/nri3216
State	Published - Jul 2012
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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@article{a0aeee8678514a3aa2cc7f6e1de1a111,

title = "Immunoglobulin class-switch DNA recombination: Induction, targeting and beyond",

abstract = "Class-switch DNA recombination (CSR) of the immunoglobulin heavy chain (IGH) locus is central to the maturation of the antibody response and crucially requires the cytidine deaminase AID. CSR involves changes in the chromatin state and the transcriptional activation of the IGH locus at the upstream and downstream switch (S) regions that are to undergo S-S DNA recombination. In addition, CSR involves the induction of AID expression and the targeting of CSR factors to S regions by 14-3-3 adaptors, and it is facilitated by the transcription machinery and by histone modifications. In this Review, we focus on recent advances regarding the induction and targeting of CSR and outline an integrated model of the assembly of macromolecular complexes that transduce crucial epigenetic information to enzymatic effectors of the CSR machinery.",

author = "Zhenming Xu and Hong Zan and Pone, {Egest J.} and Thach Mai and Paolo Casali",

note = "Funding Information: We apologize that owing to space limitations only a fraction of the relevant literature was cited. We thank all other members of the Casali laboratory for careful evaluation of this manuscript. Work on class-switch DNA recombination in the Casali laboratory has been supported by US National Institutes of Health grants AI 079705, AI 045011 and AI 060573. T. Mai has been supported by grant T32 CA 009054.",

year = "2012",

month = jul,

doi = "10.1038/nri3216",

language = "English (US)",

volume = "12",

pages = "517--531",

journal = "Nature Reviews Immunology",

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T1 - Immunoglobulin class-switch DNA recombination

T2 - Induction, targeting and beyond

AU - Xu, Zhenming

AU - Zan, Hong

AU - Pone, Egest J.

AU - Mai, Thach

AU - Casali, Paolo

N1 - Funding Information: We apologize that owing to space limitations only a fraction of the relevant literature was cited. We thank all other members of the Casali laboratory for careful evaluation of this manuscript. Work on class-switch DNA recombination in the Casali laboratory has been supported by US National Institutes of Health grants AI 079705, AI 045011 and AI 060573. T. Mai has been supported by grant T32 CA 009054.

PY - 2012/7

Y1 - 2012/7

N2 - Class-switch DNA recombination (CSR) of the immunoglobulin heavy chain (IGH) locus is central to the maturation of the antibody response and crucially requires the cytidine deaminase AID. CSR involves changes in the chromatin state and the transcriptional activation of the IGH locus at the upstream and downstream switch (S) regions that are to undergo S-S DNA recombination. In addition, CSR involves the induction of AID expression and the targeting of CSR factors to S regions by 14-3-3 adaptors, and it is facilitated by the transcription machinery and by histone modifications. In this Review, we focus on recent advances regarding the induction and targeting of CSR and outline an integrated model of the assembly of macromolecular complexes that transduce crucial epigenetic information to enzymatic effectors of the CSR machinery.

AB - Class-switch DNA recombination (CSR) of the immunoglobulin heavy chain (IGH) locus is central to the maturation of the antibody response and crucially requires the cytidine deaminase AID. CSR involves changes in the chromatin state and the transcriptional activation of the IGH locus at the upstream and downstream switch (S) regions that are to undergo S-S DNA recombination. In addition, CSR involves the induction of AID expression and the targeting of CSR factors to S regions by 14-3-3 adaptors, and it is facilitated by the transcription machinery and by histone modifications. In this Review, we focus on recent advances regarding the induction and targeting of CSR and outline an integrated model of the assembly of macromolecular complexes that transduce crucial epigenetic information to enzymatic effectors of the CSR machinery.

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Immunoglobulin class-switch DNA recombination: Induction, targeting and beyond

Abstract

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