Immunogenicity of hepatitis B surface antigen derived from the baculovirus expression vector system: a mouse potency study

Roberta Attanasio; Robert E. Lanford; Dennis Dilley; Gregory W. Stunz; Lena Notvall; A. Burl Henderson; Ronald C. Kennedy

doi:10.1016/S1045-1056(05)80024-7

Immunogenicity of hepatitis B surface antigen derived from the baculovirus expression vector system: a mouse potency study

Roberta Attanasio, Robert E. Lanford, Dennis Dilley, Gregory W. Stunz, Lena Notvall, A. Burl Henderson, Ronald C. Kennedy

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A standard mouse potency test was performed to evaluate the immunogenicity of recombinant hepatitis B surface antigen (HBsAg) produced in the baculovirus/insect cell expression system. Groups of NIH Swiss mice were immunized with serial four-fold amounts of either baculovirus-derived HBsAg adsorbed to aluminum sulfate or a commercially available yeast-derived recombinant HBsAg vaccine preparation. Results from these experiments showed that the effective dose of baculovirus-and yeast-derived HBsAg vaccine preparations necessary to seroconvert 50% of the animals were similar. The duration of the antibody response to HBsAg was studied in mice immunized with the highest doses of the two recombinant vaccine preparations 3 and 6 months after injection. No decrease in the anti-HBs response was observed 6 months after injection. No decrease in the anti-HBs response was observed 6 months after immunization with either of the two vaccine preparations. These results indicate that the baculovirus-derived recombinant HBsAg could serve as an alternative vaccine candidate for hepatitis B virus.

Original language	English (US)
Pages (from-to)	347-353
Number of pages	7
Journal	Biologicals
Volume	19
Issue number	4
DOIs	https://doi.org/10.1016/S1045-1056(05)80024-7
State	Published - Oct 1991

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Immunology and Microbiology(all)
Pharmacology

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Immunogenicity of hepatitis B surface antigen derived from the baculovirus expression vector system : a mouse potency study. / Attanasio, Roberta; Lanford, Robert E.; Dilley, Dennis; Stunz, Gregory W.; Notvall, Lena; Henderson, A. Burl; Kennedy, Ronald C.

In: Biologicals, Vol. 19, No. 4, 10.1991, p. 347-353.

Research output: Contribution to journal › Article › peer-review

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title = "Immunogenicity of hepatitis B surface antigen derived from the baculovirus expression vector system: a mouse potency study",

abstract = "A standard mouse potency test was performed to evaluate the immunogenicity of recombinant hepatitis B surface antigen (HBsAg) produced in the baculovirus/insect cell expression system. Groups of NIH Swiss mice were immunized with serial four-fold amounts of either baculovirus-derived HBsAg adsorbed to aluminum sulfate or a commercially available yeast-derived recombinant HBsAg vaccine preparation. Results from these experiments showed that the effective dose of baculovirus-and yeast-derived HBsAg vaccine preparations necessary to seroconvert 50% of the animals were similar. The duration of the antibody response to HBsAg was studied in mice immunized with the highest doses of the two recombinant vaccine preparations 3 and 6 months after injection. No decrease in the anti-HBs response was observed 6 months after injection. No decrease in the anti-HBs response was observed 6 months after immunization with either of the two vaccine preparations. These results indicate that the baculovirus-derived recombinant HBsAg could serve as an alternative vaccine candidate for hepatitis B virus.",

author = "Roberta Attanasio and Lanford, {Robert E.} and Dennis Dilley and Stunz, {Gregory W.} and Lena Notvall and Henderson, {A. Burl} and Kennedy, {Ronald C.}",

note = "Funding Information: This work was supported in part by Grants AI-22307, AI-22380, and CA-53246. The authors wish to thank Ms Jo Fletcher for the excellent assistance in the preparatmn of this manuscript.",

year = "1991",

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doi = "10.1016/S1045-1056(05)80024-7",

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AU - Dilley, Dennis

AU - Stunz, Gregory W.

AU - Notvall, Lena

AU - Henderson, A. Burl

AU - Kennedy, Ronald C.

N1 - Funding Information: This work was supported in part by Grants AI-22307, AI-22380, and CA-53246. The authors wish to thank Ms Jo Fletcher for the excellent assistance in the preparatmn of this manuscript.

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N2 - A standard mouse potency test was performed to evaluate the immunogenicity of recombinant hepatitis B surface antigen (HBsAg) produced in the baculovirus/insect cell expression system. Groups of NIH Swiss mice were immunized with serial four-fold amounts of either baculovirus-derived HBsAg adsorbed to aluminum sulfate or a commercially available yeast-derived recombinant HBsAg vaccine preparation. Results from these experiments showed that the effective dose of baculovirus-and yeast-derived HBsAg vaccine preparations necessary to seroconvert 50% of the animals were similar. The duration of the antibody response to HBsAg was studied in mice immunized with the highest doses of the two recombinant vaccine preparations 3 and 6 months after injection. No decrease in the anti-HBs response was observed 6 months after injection. No decrease in the anti-HBs response was observed 6 months after immunization with either of the two vaccine preparations. These results indicate that the baculovirus-derived recombinant HBsAg could serve as an alternative vaccine candidate for hepatitis B virus.

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Immunogenicity of hepatitis B surface antigen derived from the baculovirus expression vector system: a mouse potency study

Abstract

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