TY - JOUR
T1 - Immunodiagnosis of systemic candidiasis
T2 - Mannan antigenemia detected by radioimmunoassay in experimental and human infections
AU - Weiner, Marc H.
AU - Coats-Stephen, Marion
N1 - Funding Information:
Received for publication January 23, 1979, and in revised form June 1, 1979. This work was presented in part at the Southern Section of the American Federation of Clinical Research, New Orleans, Louisiana, January 1979. The study was supported by grant no. AI-l3nO-03 from the National Institutes of Health and by the Medical Research Service of the Veterans Administration. Candida albicans group A (B31l) was obtained from the late Dr. H. F. Hasenclever, and clinical isolates of C. albicans and Candida tropica/is from Dr. J. Johnson of the University of Texas Health Science Center. We also thank Yvonne Wylie for technical assistance. Please address requests for reprints to Dr. Marc H. Weiner, Division of Infectious Diseases, Department of Medicine, University of Texas Health Science Center, n03 Floyd Curl Drive, San Antonio, Texas 78284.
PY - 1979/12
Y1 - 1979/12
N2 - A radioimmunoassay (RIA) that detects Candida mannan was developed so that immu-nodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidiasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial Candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candidiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.
AB - A radioimmunoassay (RIA) that detects Candida mannan was developed so that immu-nodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidiasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial Candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candidiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.
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U2 - 10.1093/infdis/140.6.989
DO - 10.1093/infdis/140.6.989
M3 - Article
C2 - 94341
AN - SCOPUS:0018571998
VL - 140
SP - 989
EP - 993
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 6
ER -