Abstract
A radioimmunoassay (RIA) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidiasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candididiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 989-993 |
| Number of pages | 5 |
| Journal | Journal of Infectious Diseases |
| Volume | 140 |
| Issue number | 6 |
| State | Published - 1979 |
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ASJC Scopus subject areas
- Immunology
- Public Health, Environmental and Occupational Health
Cite this
Immunodiagnosis of systemic candidiasis : mannan antigenemia detected by radioimmunoassay in experimental and human infections. / Weiner, Marc H; Coats-Stephen, M.
In: Journal of Infectious Diseases, Vol. 140, No. 6, 1979, p. 989-993.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunodiagnosis of systemic candidiasis
T2 - mannan antigenemia detected by radioimmunoassay in experimental and human infections
AU - Weiner, Marc H
AU - Coats-Stephen, M.
PY - 1979
Y1 - 1979
N2 - A radioimmunoassay (RIA) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidiasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candididiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.
AB - A radioimmunoassay (RIA) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidiasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candididiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018571998&partnerID=8YFLogxK
M3 - Article
C2 - 94341
AN - SCOPUS:0018571998
VL - 140
SP - 989
EP - 993
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 6
ER -