Immunodiagnosis of systemic candidiasis: mannan antigenemia detected by radioimmunoassay in experimental and human infections

Marc H Weiner, M. Coats-Stephen

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A radioimmunoassay (RIA) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidiasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candididiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.

Original languageEnglish (US)
Pages (from-to)989-993
Number of pages5
JournalJournal of Infectious Diseases
Volume140
Issue number6
StatePublished - 1979

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Mannans
Immunologic Tests
Radioimmunoassay
Infection
Candida
Invasive Candidiasis
Rabbits
Serum
Candidemia
Animal Disease Models
Aspergillosis
Systemic candidiasis
Mycoses
Catheters
Tissue Donors

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Immunodiagnosis of systemic candidiasis : mannan antigenemia detected by radioimmunoassay in experimental and human infections. / Weiner, Marc H; Coats-Stephen, M.

In: Journal of Infectious Diseases, Vol. 140, No. 6, 1979, p. 989-993.

Research output: Contribution to journalArticle

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