Immunodiagnosis of systemic candidiasis: Mannan antigenemia detected by radioimmunoassay in experimental and human infections

Marc H. Weiner, Marion Coats-Stephen

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

A radioimmunoassay (RIA) that detects Candida mannan was developed so that immu-nodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidiasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial Candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candidiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.

Original languageEnglish (US)
Pages (from-to)989-993
Number of pages5
JournalJournal of Infectious Diseases
Volume140
Issue number6
DOIs
StatePublished - Dec 1979

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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