Abstract
To improve antemortem diagnosis of invasive pulmonary aspergillosis, we used a radioimmunoassay to detect an Aspergillus fumigatus antigen in bronchoalveolar lavage fluid (BALF) and evaluated this technique in a model of disseminated aspergillosis. Antigen was detected in 91% of BALF samples obtained from 11 rabbits with major pulmonary aspergillosis. In an additional 10 rabbits with only minor pulmonary involvement, antigen was detected in 40% of concentrated lavage samples. In contrast, antigenlike activity was found in only 1 of 17 BALF samples from control animals with systemic candidiasis, in none of 9 control animals with staphylococcal pneumonia and in none of 10 normal control animals. Although antigen was present in the serum of 76% of animals infected with Aspergillus, 27% of those with major pulmonary involvement had antigen detected in BALF alone. We found that an extracellular microbial antigen can be detected in BALF and that this technique in the disseminated aspergillosis model is both sensitive and specific for invasive pulmonary disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 60-64 |
| Number of pages | 5 |
| Journal | American Review of Respiratory Disease |
| Volume | 124 |
| Issue number | 1 |
| State | Published - 1981 |
Fingerprint
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Immunodiagnosis of invasive pulmonary aspergillosis in rabbits. Fungal antigen detected by radioimmunoassay in bronchoalveolar lavage fluid. / Andrews, C. P.; Weiner, Marc H.
In: American Review of Respiratory Disease, Vol. 124, No. 1, 1981, p. 60-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunodiagnosis of invasive pulmonary aspergillosis in rabbits. Fungal antigen detected by radioimmunoassay in bronchoalveolar lavage fluid
AU - Andrews, C. P.
AU - Weiner, Marc H
PY - 1981
Y1 - 1981
N2 - To improve antemortem diagnosis of invasive pulmonary aspergillosis, we used a radioimmunoassay to detect an Aspergillus fumigatus antigen in bronchoalveolar lavage fluid (BALF) and evaluated this technique in a model of disseminated aspergillosis. Antigen was detected in 91% of BALF samples obtained from 11 rabbits with major pulmonary aspergillosis. In an additional 10 rabbits with only minor pulmonary involvement, antigen was detected in 40% of concentrated lavage samples. In contrast, antigenlike activity was found in only 1 of 17 BALF samples from control animals with systemic candidiasis, in none of 9 control animals with staphylococcal pneumonia and in none of 10 normal control animals. Although antigen was present in the serum of 76% of animals infected with Aspergillus, 27% of those with major pulmonary involvement had antigen detected in BALF alone. We found that an extracellular microbial antigen can be detected in BALF and that this technique in the disseminated aspergillosis model is both sensitive and specific for invasive pulmonary disease.
AB - To improve antemortem diagnosis of invasive pulmonary aspergillosis, we used a radioimmunoassay to detect an Aspergillus fumigatus antigen in bronchoalveolar lavage fluid (BALF) and evaluated this technique in a model of disseminated aspergillosis. Antigen was detected in 91% of BALF samples obtained from 11 rabbits with major pulmonary aspergillosis. In an additional 10 rabbits with only minor pulmonary involvement, antigen was detected in 40% of concentrated lavage samples. In contrast, antigenlike activity was found in only 1 of 17 BALF samples from control animals with systemic candidiasis, in none of 9 control animals with staphylococcal pneumonia and in none of 10 normal control animals. Although antigen was present in the serum of 76% of animals infected with Aspergillus, 27% of those with major pulmonary involvement had antigen detected in BALF alone. We found that an extracellular microbial antigen can be detected in BALF and that this technique in the disseminated aspergillosis model is both sensitive and specific for invasive pulmonary disease.
UR - http://www.scopus.com/inward/record.url?scp=0019406035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019406035&partnerID=8YFLogxK
M3 - Article
C2 - 7020504
AN - SCOPUS:0019406035
VL - 124
SP - 60
EP - 64
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
SN - 1073-449X
IS - 1
ER -