Immunocytochemical identification of rat growth hormone cells utilizing antisera to nonmammalian growth hormones

John R. McGill; Damon C. Herbert; Ted Hayashida

doi:10.1016/0016-6480(78)90080-1

Immunocytochemical identification of rat growth hormone cells utilizing antisera to nonmammalian growth hormones

John R. McGill, Damon C. Herbert, Ted Hayashida

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

An attempt was made to identify rat pituitary growth hormone cells by immunocytochemical methods utilizing monkey antisera to shark growth hormone, snapping turtle growth hormone, and rat growth hormone. The localization of rat growth hormone at the light microscope level was achieved using a modification of the unlabeled antibody enzyme method of Sternberger. Specific staining was observed in cells that were ovoid or pyramidal in shape which were generally evenly distributed across the entire section.

Original language	English (US)
Pages (from-to)	342-345
Number of pages	4
Journal	General and Comparative Endocrinology
Volume	35
Issue number	3
DOIs	https://doi.org/10.1016/0016-6480(78)90080-1
State	Published - Jul 1978

ASJC Scopus subject areas

Animal Science and Zoology
Endocrinology

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@article{81ebfdf3d9f344b79b06c9640f9fe5d5,

title = "Immunocytochemical identification of rat growth hormone cells utilizing antisera to nonmammalian growth hormones",

abstract = "An attempt was made to identify rat pituitary growth hormone cells by immunocytochemical methods utilizing monkey antisera to shark growth hormone, snapping turtle growth hormone, and rat growth hormone. The localization of rat growth hormone at the light microscope level was achieved using a modification of the unlabeled antibody enzyme method of Sternberger. Specific staining was observed in cells that were ovoid or pyramidal in shape which were generally evenly distributed across the entire section.",

author = "McGill, {John R.} and Herbert, {Damon C.} and Ted Hayashida",

note = "Funding Information: The authors wish to thank Martha Castilleja and Pauline Cisneros for their excellent technical assistance. Sincere appreciation is also extended to the NIAMDD for providing us with the rat prolactin and bovine GH preparations and to Dr. Ludwig Sternberger for the PAP complex. The project was supported in part by NIH grants AM12583 and HD-04063 (T.H.).",

year = "1978",

month = jul,

doi = "10.1016/0016-6480(78)90080-1",

language = "English (US)",

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pages = "342--345",

journal = "General and Comparative Endocrinology",

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T1 - Immunocytochemical identification of rat growth hormone cells utilizing antisera to nonmammalian growth hormones

AU - McGill, John R.

AU - Herbert, Damon C.

AU - Hayashida, Ted

N1 - Funding Information: The authors wish to thank Martha Castilleja and Pauline Cisneros for their excellent technical assistance. Sincere appreciation is also extended to the NIAMDD for providing us with the rat prolactin and bovine GH preparations and to Dr. Ludwig Sternberger for the PAP complex. The project was supported in part by NIH grants AM12583 and HD-04063 (T.H.).

PY - 1978/7

Y1 - 1978/7

N2 - An attempt was made to identify rat pituitary growth hormone cells by immunocytochemical methods utilizing monkey antisera to shark growth hormone, snapping turtle growth hormone, and rat growth hormone. The localization of rat growth hormone at the light microscope level was achieved using a modification of the unlabeled antibody enzyme method of Sternberger. Specific staining was observed in cells that were ovoid or pyramidal in shape which were generally evenly distributed across the entire section.

AB - An attempt was made to identify rat pituitary growth hormone cells by immunocytochemical methods utilizing monkey antisera to shark growth hormone, snapping turtle growth hormone, and rat growth hormone. The localization of rat growth hormone at the light microscope level was achieved using a modification of the unlabeled antibody enzyme method of Sternberger. Specific staining was observed in cells that were ovoid or pyramidal in shape which were generally evenly distributed across the entire section.

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JF - General and Comparative Endocrinology

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ER -

Immunocytochemical identification of rat growth hormone cells utilizing antisera to nonmammalian growth hormones

Abstract

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