Abstract
Actin like protein, extracted and purified from Vigna radiata (mung bean) seedling, has been found to give positive enzyme-linked immunosorbent assay with mouse monoclonal antiactin antibody. In vivo studies show that cytochalasin B at sublethal dose inhibits the chromosomal movement at metaphase stage during germination. From in vitro studies it is found that the actin like protein isolated from mung bean seedling has a cytochalasin B binding property with a Kd value 1.2 × 10-5 M. From these two specific observations it appears probable that the biological function of mung bean actin like protein is to take part in cell division process directly or indirectly during the time of seedling development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 209-219 |
| Number of pages | 11 |
| Journal | Journal of Biosciences |
| Volume | 14 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 1989 |
| Externally published | Yes |
Keywords
- ELISA
- Vigna radiata
- cytochalasin B
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
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Cite this
Ghosh, G., Pal, A., & Biswas, S. (1989). Immunochemical identification of mung bean actin like protein and its cellular involvement during germination. Journal of Biosciences, 14(3), 209-219. https://doi.org/10.1007/BF02716681