Immunochemical characterization and quantitation of lipoprotein (a) in baboons. Development of an assay depending on two antigenically distinct proteins

David L. Rainwater, G. Scott Manis

    Research output: Contribution to journalArticlepeer-review

    29 Scopus citations

    Abstract

    We have raised specific antibodies against the protein component of baboon lipoprotein (a) (Lp(a)). Apolipoprotein (apo) Lp(a) is a very large protein which separates into two distinct proteins, apo B and apo (a), when 2-mercaptoethanol is included during sample treatment for sodium dodecyl sulfate-electrophoresis. The antibodies were specific for baboon apo (a) and apo B. The presence of the two distinct antigens in the lipoprotein permitted the development of an enzyme-linked immunosorbent assay that was specific for Lp(a) particles in serum. The assay could detect less than 1 ng of Lp(a) protein per well and was useful in the range of 1-9 ng. The assay was specific for Lp(a) and did not respond to other lipoproteins, such as low density lipoprotein. Lp(a) could be accurately quantitated in serum frozen at -80 ° C in plastic tubing segments. Using the Lp(a) assay, the mean serum level of 80 unrelated baboons was 4.7 mg/dl, with the distribution skewed toward the lower levels. These data further support the value of the baboon as a model of the atherogenic lipoprotein Lp(a).

    Original languageEnglish (US)
    Pages (from-to)23-31
    Number of pages9
    JournalAtherosclerosis
    Volume73
    Issue number1
    DOIs
    StatePublished - Sep 1988

    Keywords

    • (Baboon)
    • Apolipoprotein Lp(a)
    • Lipoprotein (a)

    ASJC Scopus subject areas

    • Cardiology and Cardiovascular Medicine

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