Immunization with dendritic cells pulsed ex vivo with recombinant chlamydial protease-like activity factor induces protective immunity against genital chlamydia muridarum challenge

Weidang Li, Ashlesh K. Murthy, B. K R Chaganty, M. Neal Guentzel, J. Seshu, James P. Chambers, Guangming Zhong, Bernard P. Arulanandam

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have shown that immunization with soluble recombinant chlamydial protease-like activity factor (rCPAF) and a T helper 1 type adjuvant can induce significantly enhanced bacterial clearance and protection against Chlamydia-induced pathological sequelae in the genital tract. In this study, we investigated the use of bone marrow derived dendritic cells (BMDCs) pulsed ex vivo with rCPAF + CpG in an adoptive subcutaneous immu-nization for the ability to induce protective immunity against genital chlamydial infection. We found that BMDCs pulsed with rCPAF + CpG efficiently up-regulated the expression of activation markers CD86, CD80, CD40, and major histocompatibility complex class II (MHC II), and secreted interleukin-12, but not IL-10 and IL-4. Mice adoptively immunized with rCPAF + CpG-pulsed BMDCs or UV-EB + CpG-pulsed BMDCs produced elevated lev-els of antigen-specific IFN-γ and enhanced IgG1 and IgG2a antibodies. Moreover, mice immunized with rCPAF + CpG-pulsed BMDCs or UV-EB + CpG-pulsed BMDCs exhibited significantly reduced genital Chlamydia shedding, accelerated resolution of infection, and reduced oviduct pathology when compared to infected mock-immunized animals. These results suggest that adoptive subcutaneous immunization with ex vivo rCPAF-pulsed BMDCs is an effective approach, comparable to that induced by UV-EB-BMDCs, for inducing robust anti-Chlamydia immunity.

Original languageEnglish (US)
Article numberArticle 73
JournalFrontiers in Immunology
Volume2
Issue numberDEC
DOIs
StatePublished - 2011

Fingerprint

Chlamydia muridarum
Dendritic Cells
Immunity
Immunization
Peptide Hydrolases
Bone Marrow
Chlamydia
Oviducts
Protective Factors
Interleukin-12
Infection
Major Histocompatibility Complex
Interleukin-1
Interleukin-4
Interleukin-10
Immunoglobulin G
Pathology

Keywords

  • Chlamydia trachomatis
  • CPAF
  • Dendritic cell
  • Genital infection
  • UV-EB

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Immunization with dendritic cells pulsed ex vivo with recombinant chlamydial protease-like activity factor induces protective immunity against genital chlamydia muridarum challenge. / Li, Weidang; Murthy, Ashlesh K.; Chaganty, B. K R; Guentzel, M. Neal; Seshu, J.; Chambers, James P.; Zhong, Guangming; Arulanandam, Bernard P.

In: Frontiers in Immunology, Vol. 2, No. DEC, Article 73, 2011.

Research output: Contribution to journalArticle

Li, Weidang ; Murthy, Ashlesh K. ; Chaganty, B. K R ; Guentzel, M. Neal ; Seshu, J. ; Chambers, James P. ; Zhong, Guangming ; Arulanandam, Bernard P. / Immunization with dendritic cells pulsed ex vivo with recombinant chlamydial protease-like activity factor induces protective immunity against genital chlamydia muridarum challenge. In: Frontiers in Immunology. 2011 ; Vol. 2, No. DEC.
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