Immunization of mice with baculovirus-derived recombinant SV40 large tumour antigen induces protective tumour immunity to a lethal challenge with SV40-transformed cells

M. H. Shearer, R. K. Bright, R. E. Lanford, R. C. Kennedy

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

In this study, we examine the humoral immune responses and in vivo tumour immunity induced by baculovirus recombinant simian virus 40 (SV40) large tumour antigen (rSV40 T-ag). BALB/c mice immunized with rSV40 T-ag produced antibody responses that recognized SV40 large tumour antigen (T-ag) by ELISA. Analysis of these anti-SV40 T-ag responses indicated that the antibodies recognized epitopes associated with both the carboxy and amino terminus of SV40 T-ag. This pattern of SV40 T-ag epitope recognition was similar to that observed in anti-SV40 T-ag responses induced by inoculation with irradiated SV40-transformed cells. Mice immunized with either rSV40 T-ag or with the inactivated transformed cells were protected from a subsequent in vivo lethal tumour challenge with live SV40-transformed cells. These studies suggest that humoral immune responses induced by rSV40 T-ag are similar in epitope specificity to that induced by inactivated SV40-transformed cells. In addition, recombinant tumour-specific antigens from papovaviruses, such as SV40, can be used to induce tumour immunity which protects from a subsequent lethal tumour challenge. This study may provide insight into the use of recombinant tumour antigens as putative tumour vaccines and in the development of active immunotherapeutic strategies for treating virus-induced cancers.

Original languageEnglish (US)
Pages (from-to)266-271
Number of pages6
JournalClinical and Experimental Immunology
Volume91
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • recombinant tumour antigen
  • tumour vaccine
  • virus induced tumours

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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