Immune response of male baboons to testis-specific LDH-C4

Erwin Goldberg, John L. VandeBerg, Mary C. Mahony, Gustavo F. Doncel

    Research output: Contribution to journalArticle

    17 Scopus citations

    Abstract

    Four sexually mature male baboons (Papio sp.) were immunized with a chimeric peptide containing a B-cell epitope of the testis-specific lactate dehydrogenase (LDH-C4) and a promiscuous T-cell epitope of tetanus toxin. LDH-C4 is the testis-specific isozyme of lactate dehydrogenase, and antibodies to this protein reduce fertility significantly in female nonhuman primates. Animals were immunized on Day 0 and received booster injections at Days 29, 61, and 344 after priming. Serum specific antibodies were determined at regular intervals during the initial 6 months and after the last booster. Testis biopsies were taken at Days 61, 127, and 183 after the primary immunization. Sperm-zona binding was assessed prior to and three times after the last booster. The present study demonstrated that this epitope of LDH-C4 did not cause autoimmune disease and that sperm from these immunized males had a diminished zona binding capacity. These results suggest that a safe male immunocontraceptive based on development of anti-sperm antibodies may be feasible.

    Original languageEnglish (US)
    Pages (from-to)93-98
    Number of pages6
    JournalContraception
    Volume64
    Issue number2
    DOIs
    StatePublished - Jan 1 2001

    Keywords

    • Autoimmunity
    • Baboon
    • Immunocontraception
    • LDH-C
    • Male contraceptive

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Obstetrics and Gynecology

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  • Cite this

    Goldberg, E., VandeBerg, J. L., Mahony, M. C., & Doncel, G. F. (2001). Immune response of male baboons to testis-specific LDH-C4 Contraception, 64(2), 93-98. https://doi.org/10.1016/S0010-7824(01)00227-X