Immediately preovulatory levels of messenger ribonucleic acid for inhibin alpha-subunit are diminished in granulosa cells from successful in-vitro fertilization-embryo transfer

William N. Burns, Rajeshwar R Tekmal, David L. Olive, Jennifer D. Jacobs, Robert M. Riehl, Robert S Schenken

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Inhibin, a gonadal glycoprotem which suppresses pituitary gonadotrophin secretion, preferentially follicle stimulating hormone, has been extensively characterized. It consists of two covalently bound subunits, the α- and β-subunits, encoded by separate genes. In this study, the expression of messenger ribonucleic acid (mRNA) for the inhibin α-subunit was studied by Northern blot analysis in granulosa cells of women undergoing in-vitro fertilization/embryo transfer (IVF/ET). Three patient groups were studied: women who failed to become pregnant (n=11), women who became pregnant but experienced early spontaneous abortion (n=3) and women who conceived normal ongoing pregnancies (n=4). Granulosa cells were obtained at the time of follicle aspiration. Levels of alpha-subunit mRNA were 40% lower in patients establishing normal pregnancies than in those who failed to become pregnant or who spontaneously aborted. Thus, a relative diminution of immediately preovulatory levels of mRNA for inhibin α-subunit Is a marker of success in clinical LVFIET cycles. This marker of IVF/ET success can be related to previously established markers of success (increased follicular fluid oestradiol and decreased follicular fluid cyclic adenosine monophosphate) by known physiological mechanisms.

Original languageEnglish (US)
Pages (from-to)351-355
Number of pages5
JournalHuman Reproduction
Volume6
Issue number3
StatePublished - Mar 1991

Fingerprint

Follicular Fluid
Embryo Transfer
In Vitro Fertilization
Granulosa Cells
Pregnancy
Fertilization in Vitro
RNA
Inhibins
Immediately
Estradiol
Gonadotropins
Adenosine
Secretion
Cell
Hormones
Fluids
Gene
Pituitary Gonadotropins
Cycle
Follicle Stimulating Hormone

Keywords

  • Alpha-subunit
  • Inhibin
  • IVF-ET
  • Messenger ribonucleic acid

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Rehabilitation
  • Physiology
  • Developmental Biology

Cite this

Immediately preovulatory levels of messenger ribonucleic acid for inhibin alpha-subunit are diminished in granulosa cells from successful in-vitro fertilization-embryo transfer. / Burns, William N.; Tekmal, Rajeshwar R; Olive, David L.; Jacobs, Jennifer D.; Riehl, Robert M.; Schenken, Robert S.

In: Human Reproduction, Vol. 6, No. 3, 03.1991, p. 351-355.

Research output: Contribution to journalArticle

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abstract = "Inhibin, a gonadal glycoprotem which suppresses pituitary gonadotrophin secretion, preferentially follicle stimulating hormone, has been extensively characterized. It consists of two covalently bound subunits, the α- and β-subunits, encoded by separate genes. In this study, the expression of messenger ribonucleic acid (mRNA) for the inhibin α-subunit was studied by Northern blot analysis in granulosa cells of women undergoing in-vitro fertilization/embryo transfer (IVF/ET). Three patient groups were studied: women who failed to become pregnant (n=11), women who became pregnant but experienced early spontaneous abortion (n=3) and women who conceived normal ongoing pregnancies (n=4). Granulosa cells were obtained at the time of follicle aspiration. Levels of alpha-subunit mRNA were 40{\%} lower in patients establishing normal pregnancies than in those who failed to become pregnant or who spontaneously aborted. Thus, a relative diminution of immediately preovulatory levels of mRNA for inhibin α-subunit Is a marker of success in clinical LVFIET cycles. This marker of IVF/ET success can be related to previously established markers of success (increased follicular fluid oestradiol and decreased follicular fluid cyclic adenosine monophosphate) by known physiological mechanisms.",
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