Imaging cocaine-induced changes in the mesocorticolimbic dopaminergic system of conscious rats

Marcelo Febo, Annabell C. Segarra, Jeffrey R. Tenney, Mathew E. Brevard, Timothy Q. Duong, Craig F. Ferris

Research output: Contribution to journalArticle

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Abstract

Functional magnetic resonance imaging (fMRI) was used to assess the effects of cocaine on brain activation in fully conscious rats. Methods were developed to image cocaine-induced changes in blood-oxygen-level-dependent (BOLD) signal without the peripheral cardiac and respiratory complications associated with psychostimulant administration. Using spin echo planar imaging (EPI), conscious rats were imaged in a 4.7 T spectrometer prior to and following the intracerebroventricular injection of cocaine (20 μg) in artificial cerebrospinal fluid (10 uL). Within 5 min of injection, there was a significant increase in BOLD signal intensity in the substantia nigra, ventral tegmental area, nucleus accumbens, dorsal striatum and prefrontal cortex, as compared to vehicle controls. Minimal negative BOLD signal changes were observed in response to cocaine and no significant perturbations in normal cardiovascular and respiratory function. These findings demonstrate the technical feasibility of studying psychostimulant-induced brain activity using functional MRI in conscious rats.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalJournal of Neuroscience Methods
Volume139
Issue number2
DOIs
StatePublished - Oct 30 2004
Externally publishedYes

Fingerprint

Cocaine
Magnetic Resonance Imaging
Oxygen
Tegmentum Mesencephali
Echo-Planar Imaging
Ventral Tegmental Area
Injections
Nucleus Accumbens
Brain
Substantia Nigra
Prefrontal Cortex
Cerebrospinal Fluid

Keywords

  • Animal imaging
  • BOLD technique
  • Functional imaging
  • Nucleus accumbens
  • Prefrontal cortex
  • Psychostimulants
  • Reward system

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Febo, M., Segarra, A. C., Tenney, J. R., Brevard, M. E., Duong, T. Q., & Ferris, C. F. (2004). Imaging cocaine-induced changes in the mesocorticolimbic dopaminergic system of conscious rats. Journal of Neuroscience Methods, 139(2), 167-176. https://doi.org/10.1016/j.jneumeth.2004.04.028

Imaging cocaine-induced changes in the mesocorticolimbic dopaminergic system of conscious rats. / Febo, Marcelo; Segarra, Annabell C.; Tenney, Jeffrey R.; Brevard, Mathew E.; Duong, Timothy Q.; Ferris, Craig F.

In: Journal of Neuroscience Methods, Vol. 139, No. 2, 30.10.2004, p. 167-176.

Research output: Contribution to journalArticle

Febo, Marcelo ; Segarra, Annabell C. ; Tenney, Jeffrey R. ; Brevard, Mathew E. ; Duong, Timothy Q. ; Ferris, Craig F. / Imaging cocaine-induced changes in the mesocorticolimbic dopaminergic system of conscious rats. In: Journal of Neuroscience Methods. 2004 ; Vol. 139, No. 2. pp. 167-176.
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