IL-12 enhances antibody responses to T-independent polysaccharide vaccines in the absence of T and NK cells

Renee M. Buchanan, Bernard P. Arulanandam, Dennis W. Metzger

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Polysaccharide vaccines to encapsulated bacteria such as Neisseria meningitidis and streptococcus pneumoniae are weakly immunogenic due to their T-independent (TI) nature. Even when converted to T-dependent forms through conjugation to foreign proteins, polysaccharides induce responses that are deficient in many respects, such as induction of murine IgG2a Ab, the isotype that mediates optimal complement fixation and opsonization. We now show that IL-12 treatment of mice induces significantly increased levels of IgG2a Ab to the model TI-2 Ag, DNP-Ficoll, and to vaccines composed of polysaccharides from pneumococci and meningococci. Use of immunodeficient mice lacking T cells and/or NK cells demonstrated that such cells were not responsible for the observed Ab enhancement. Furthermore, the use of IFN-γ knockout mice showed that stimulation of TI-2 Ab responses by IL-12 was only partially dependent on IFN-γ. The ability of IL-12 to dramatically enhance TI Ab responses suggests that IL-12 will be useful as a powerful vaccine adjuvant to induce protective immune responses against encapsulated pathogens.

Original languageEnglish (US)
Pages (from-to)5525-5533
Number of pages9
JournalJournal of Immunology
Volume161
Issue number10
StatePublished - Nov 15 1998
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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