IL-10通过抑制IFN-γ和IL-2表达促进鼠衣原体感染

Translated title of the contribution: IL-10 promotes Chlamydia muridarum infection in mice through inhibiting IFN-γ and IL-2 expression

Qingzhi He, Mengting He, Xin Wang, Xiaoqing Luo, Yimou Wu, Guangming Zhong

Research output: Contribution to journalArticle

Abstract

Objective: To study the possible molecular mechanism of IL-10 in promoting Chlamydia muridarum infection in mice. Methods: C57BL/6 wild-type and IL-10 gene knockout (IL-10-/-) mice were infected with Chlamydia muridarum. Indirect immunofluorescence assay was used to detect the growth of Chlamydia muridarum in the intestinal and genital tracts. The severity of genital diseases was assessed by hydrosalpinx scoring. Expression of IFN-γ and IL-2 in blood was measured by ELISA. Results: Compared with the wild-type group, Chlamydia clearance in the intestinal and genital tracts of IL-10-/- mice was significantly faster, and the expression of IFN-γ and IL-2 increased significantly. In addition, wild-type mice showed more serious hydrosalpinx. Conclusions: IL-10 delays Chlamydia trachomatis clearance and promotes Chlamydia infection through inhibiting the expression of IFN-γ and IL-2, which aggravates hydrosalpinx.

Original languageChinese
Pages (from-to)126-130
Number of pages5
JournalChinese Journal of Microbiology and Immunology (China)
Volume39
Issue number2
DOIs
StatePublished - Feb 28 2019

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Chlamydia muridarum
Chlamydia Infections
Interleukin-10
Interleukin-2
Gene Knockout Techniques
Chlamydia
Chlamydia trachomatis
Indirect Fluorescent Antibody Technique
Enzyme-Linked Immunosorbent Assay
Growth

Keywords

  • Chlamydia trachomatis
  • Cytokine
  • IL-10
  • Immunity

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

IL-10通过抑制IFN-γ和IL-2表达促进鼠衣原体感染. / He, Qingzhi; He, Mengting; Wang, Xin; Luo, Xiaoqing; Wu, Yimou; Zhong, Guangming.

In: Chinese Journal of Microbiology and Immunology (China), Vol. 39, No. 2, 28.02.2019, p. 126-130.

Research output: Contribution to journalArticle

He, Qingzhi ; He, Mengting ; Wang, Xin ; Luo, Xiaoqing ; Wu, Yimou ; Zhong, Guangming. / IL-10通过抑制IFN-γ和IL-2表达促进鼠衣原体感染. In: Chinese Journal of Microbiology and Immunology (China). 2019 ; Vol. 39, No. 2. pp. 126-130.
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abstract = "Objective: To study the possible molecular mechanism of IL-10 in promoting Chlamydia muridarum infection in mice. Methods: C57BL/6 wild-type and IL-10 gene knockout (IL-10-/-) mice were infected with Chlamydia muridarum. Indirect immunofluorescence assay was used to detect the growth of Chlamydia muridarum in the intestinal and genital tracts. The severity of genital diseases was assessed by hydrosalpinx scoring. Expression of IFN-γ and IL-2 in blood was measured by ELISA. Results: Compared with the wild-type group, Chlamydia clearance in the intestinal and genital tracts of IL-10-/- mice was significantly faster, and the expression of IFN-γ and IL-2 increased significantly. In addition, wild-type mice showed more serious hydrosalpinx. Conclusions: IL-10 delays Chlamydia trachomatis clearance and promotes Chlamydia infection through inhibiting the expression of IFN-γ and IL-2, which aggravates hydrosalpinx.",
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AB - Objective: To study the possible molecular mechanism of IL-10 in promoting Chlamydia muridarum infection in mice. Methods: C57BL/6 wild-type and IL-10 gene knockout (IL-10-/-) mice were infected with Chlamydia muridarum. Indirect immunofluorescence assay was used to detect the growth of Chlamydia muridarum in the intestinal and genital tracts. The severity of genital diseases was assessed by hydrosalpinx scoring. Expression of IFN-γ and IL-2 in blood was measured by ELISA. Results: Compared with the wild-type group, Chlamydia clearance in the intestinal and genital tracts of IL-10-/- mice was significantly faster, and the expression of IFN-γ and IL-2 increased significantly. In addition, wild-type mice showed more serious hydrosalpinx. Conclusions: IL-10 delays Chlamydia trachomatis clearance and promotes Chlamydia infection through inhibiting the expression of IFN-γ and IL-2, which aggravates hydrosalpinx.

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