Abstract
Objective: To study the possible molecular mechanism of IL-10 in promoting Chlamydia muridarum infection in mice. Methods: C57BL/6 wild-type and IL-10 gene knockout (IL-10-/-) mice were infected with Chlamydia muridarum. Indirect immunofluorescence assay was used to detect the growth of Chlamydia muridarum in the intestinal and genital tracts. The severity of genital diseases was assessed by hydrosalpinx scoring. Expression of IFN-γ and IL-2 in blood was measured by ELISA. Results: Compared with the wild-type group, Chlamydia clearance in the intestinal and genital tracts of IL-10-/- mice was significantly faster, and the expression of IFN-γ and IL-2 increased significantly. In addition, wild-type mice showed more serious hydrosalpinx. Conclusions: IL-10 delays Chlamydia trachomatis clearance and promotes Chlamydia infection through inhibiting the expression of IFN-γ and IL-2, which aggravates hydrosalpinx.
| Translated title of the contribution | IL-10 promotes Chlamydia muridarum infection in mice through inhibiting IFN-γ and IL-2 expression |
|---|---|
| Original language | Chinese (Traditional) |
| Pages (from-to) | 126-130 |
| Number of pages | 5 |
| Journal | Chinese Journal of Microbiology and Immunology (China) |
| Volume | 39 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 28 2019 |
Keywords
- Chlamydia trachomatis
- Cytokine
- IL-10
- Immunity
ASJC Scopus subject areas
- Microbiology
- Immunology
- Virology