IgG2a and IgA co-expression by the natural autoantibody-producing murine B lymphoma T560

J. M. Phillips-Quagliata, A. M.C. Faria, J. Han, D. H. Spencer, G. Haughton, P. Casali

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The T560 B lymphoma produces polyreactive IgG2a with the features of natural autoantibody. All T560 cells bear and secrete IgG2a but a small fraction spontaneously co-express IgA. Cells secreting IgA alone cannot be detected. IgA secretion is enhanced by interaction of T560 cells either with activated T cells and cognate antigen, or with LPS, but not with cytokines, including IL-5 and TGF-β. IgA and IgG2a mRNAs have identical V186.2, DFL 16 and JH1 sequences from framework 2 through JH1. PCR analysis reveals that previous recombination events have led to deletion of the μ, γ3, γ1, γ2b constant region genes from both the productive and the unproductive chromosome but the former has retained γ2a, ε and α, the latter only α. Digestion-circularization (DC)-PCR experiments provide formal proof of DNA recombination between Cα and the intron upstream of Cμ. Evidently, the productive chromosome has switched only as far as γ2a, the unproductive all the way to the α constant region gene. The unproductive allele is transcriptionally active as evidenced by the presence of mRNA encoding Cα1 inappropriately spliced to a cryptic splice site in the downstream intron of DQ52 (eliminated from the productive chromosome). A specific RT-PCR using oligonucleotide primers derived from the upstream initiation site of the Iα exon and from Cα1 discloses that T560 cells contain α-germ line mRNA, presumably transcribed from the Iα-region of the productive chromosome, spliced to Cα. Treatment with LPS stops production of these spliced transcripts suggesting that it may promote either DNA recombination in cells spontaneously transcribing Iα or a change in splicing such that Iα sequence is no longer joined to Cα. Verification of the DC-PCR product by sequencing reveals that the T560 and B10.A IgA (Ig2b allotype) hinge is different from the BALB/c IgA (Ig2a allotype) hinge: it has two extra Cys and has eliminated the first Thr, a potential glycosylation site in BALB/c IgA.

Original languageEnglish (US)
Pages (from-to)181-197+I
JournalAutoimmunity
Volume33
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • B lymphoma
  • GVH
  • IgA
  • Isotype switching

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Phillips-Quagliata, J. M., Faria, A. M. C., Han, J., Spencer, D. H., Haughton, G., & Casali, P. (2001). IgG2a and IgA co-expression by the natural autoantibody-producing murine B lymphoma T560. Autoimmunity, 33(3), 181-197+I. https://doi.org/10.3109/08916930109008046