IGF2BP3 Modulates the Interaction of Invasion-Associated Transcripts with RISC

Hanane Ennajdaoui; Jonathan M. Howard; Timothy Sterne-Weiler; Fereshteh Jahanbani; Doyle J. Coyne; Philip J. Uren; Marija Dargyte; Sol Katzman; Jolene M. Draper; Andrew Wallace; Oscar Cazarez; Suzanne C. Burns; Mei Qiao; Lindsay Hinck; Andrew D. Smith; Masoud M. Toloue; Benjamin J. Blencowe; Luiz O.F. Penalva; Jeremy R. Sanford

doi:10.1016/j.celrep.2016.04.083

IGF2BP3 Modulates the Interaction of Invasion-Associated Transcripts with RISC

Hanane Ennajdaoui, Jonathan M. Howard, Timothy Sterne-Weiler, Fereshteh Jahanbani, Doyle J. Coyne, Philip J. Uren, Marija Dargyte, Sol Katzman, Jolene M. Draper, Andrew Wallace, Oscar Cazarez, Suzanne C. Burns, Mei Qiao, Lindsay Hinck, Andrew D. Smith, Masoud M. Toloue, Benjamin J. Blencowe, Luiz O.F. Penalva, Jeremy R. Sanford

Cell Systems & Anatomy

Research output: Contribution to journal › Article

26 Scopus citations

Abstract

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) expression correlates with malignancy, but its role(s) in pathogenesis remains enigmatic. We interrogated the IGF2BP3-RNA interaction network in pancreatic ductal adenocarcinoma (PDAC) cells. Using a combination of genome-wide approaches, we have identified 164 direct mRNA targets of IGF2BP3. These transcripts encode proteins enriched for functions such as cell migration, proliferation, and adhesion. Loss of IGF2BP3 reduced PDAC cell invasiveness and remodeled focal adhesion junctions. Individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) revealed significant overlap of IGF2BP3 and microRNA (miRNA) binding sites. IGF2BP3 promotes association of the RNA-induced silencing complex (RISC) with specific transcripts. Our results show that IGF2BP3 influences a malignancy-associated RNA regulon by modulating miRNA-mRNA interactions.

Original language	English (US)
Pages (from-to)	1876-1883
Number of pages	8
Journal	Cell Reports
Volume	15
Issue number	9
DOIs	https://doi.org/10.1016/j.celrep.2016.04.083
State	Published - May 31 2016

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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Ennajdaoui, H., Howard, J. M., Sterne-Weiler, T., Jahanbani, F., Coyne, D. J., Uren, P. J., Dargyte, M., Katzman, S., Draper, J. M., Wallace, A., Cazarez, O., Burns, S. C., Qiao, M., Hinck, L., Smith, A. D., Toloue, M. M., Blencowe, B. J., Penalva, L. O. F., & Sanford, J. R. (2016). IGF2BP3 Modulates the Interaction of Invasion-Associated Transcripts with RISC. Cell Reports, 15(9), 1876-1883. https://doi.org/10.1016/j.celrep.2016.04.083

IGF2BP3 Modulates the Interaction of Invasion-Associated Transcripts with RISC. / Ennajdaoui, Hanane; Howard, Jonathan M.; Sterne-Weiler, Timothy; Jahanbani, Fereshteh; Coyne, Doyle J.; Uren, Philip J.; Dargyte, Marija; Katzman, Sol; Draper, Jolene M.; Wallace, Andrew; Cazarez, Oscar; Burns, Suzanne C.; Qiao, Mei; Hinck, Lindsay; Smith, Andrew D.; Toloue, Masoud M.; Blencowe, Benjamin J.; Penalva, Luiz O.F.; Sanford, Jeremy R.

In: Cell Reports, Vol. 15, No. 9, 31.05.2016, p. 1876-1883.

Research output: Contribution to journal › Article

Ennajdaoui, H, Howard, JM, Sterne-Weiler, T, Jahanbani, F, Coyne, DJ, Uren, PJ, Dargyte, M, Katzman, S, Draper, JM, Wallace, A, Cazarez, O, Burns, SC, Qiao, M, Hinck, L, Smith, AD, Toloue, MM, Blencowe, BJ, Penalva, LOF & Sanford, JR 2016, 'IGF2BP3 Modulates the Interaction of Invasion-Associated Transcripts with RISC', Cell Reports, vol. 15, no. 9, pp. 1876-1883. https://doi.org/10.1016/j.celrep.2016.04.083

Ennajdaoui, Hanane ; Howard, Jonathan M. ; Sterne-Weiler, Timothy ; Jahanbani, Fereshteh ; Coyne, Doyle J. ; Uren, Philip J. ; Dargyte, Marija ; Katzman, Sol ; Draper, Jolene M. ; Wallace, Andrew ; Cazarez, Oscar ; Burns, Suzanne C. ; Qiao, Mei ; Hinck, Lindsay ; Smith, Andrew D. ; Toloue, Masoud M. ; Blencowe, Benjamin J. ; Penalva, Luiz O.F. ; Sanford, Jeremy R. / IGF2BP3 Modulates the Interaction of Invasion-Associated Transcripts with RISC. In: Cell Reports. 2016 ; Vol. 15, No. 9. pp. 1876-1883.

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abstract = "Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) expression correlates with malignancy, but its role(s) in pathogenesis remains enigmatic. We interrogated the IGF2BP3-RNA interaction network in pancreatic ductal adenocarcinoma (PDAC) cells. Using a combination of genome-wide approaches, we have identified 164 direct mRNA targets of IGF2BP3. These transcripts encode proteins enriched for functions such as cell migration, proliferation, and adhesion. Loss of IGF2BP3 reduced PDAC cell invasiveness and remodeled focal adhesion junctions. Individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) revealed significant overlap of IGF2BP3 and microRNA (miRNA) binding sites. IGF2BP3 promotes association of the RNA-induced silencing complex (RISC) with specific transcripts. Our results show that IGF2BP3 influences a malignancy-associated RNA regulon by modulating miRNA-mRNA interactions.",

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AU - Wallace, Andrew

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AU - Burns, Suzanne C.

AU - Qiao, Mei

AU - Hinck, Lindsay

AU - Smith, Andrew D.

AU - Toloue, Masoud M.

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AU - Penalva, Luiz O.F.

AU - Sanford, Jeremy R.

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