IGF-I regulated phosphorylation and translocation of PDK-1 in neurons

Betty B. Alajajian, Lauren Fletcher, Elif Isgor, David F. Jimenez, Murat Digicaylioglu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

3′-Phosphoinositide-dependent protein kinase-1 (PDK-1) is a crucial serine/threonine kinase in the insulin-like growth factor-I (IGF-I)/AKT signaling pathway, but its function and localization in the nervous system has not been fully characterized. In this study, we compared the localization of PDK-1 in adult neurons and non-neuronal PC-3 cells. We showed that PC-3 cells expressed phosphorylated and nonphosphorylated PDK-1 in the cytoplasm and nucleoplasm. In contrast, neuronal PDK-1 was located in the nucleoplasm and the phosphorylated form was located along the perinuclear region. Furthermore, we found that IGF-I transiently increased phosphorylation of neuronal PDK-1, resulting in its translocation to other cellular compartments. Our findings suggest that IGF-I may regulate neuronal PDK-1 differently than in non-neuronal cells, which may indicate a novel role for PDK-1 in IGF-I-mediated neuroprotective signaling.

Original languageEnglish (US)
Pages (from-to)579-583
Number of pages5
JournalNeuroReport
Volume20
Issue number6
DOIs
StatePublished - Apr 22 2009

Fingerprint

3-Phosphoinositide-Dependent Protein Kinases
Insulin-Like Growth Factor I
Phosphorylation
Neurons
Protein-Serine-Threonine Kinases
Nervous System
Cytoplasm

Keywords

  • 3'-Phosphoinositide-dependent protein kinase-1
  • AGC kinase
  • AKT
  • Apoptosis
  • Insulin-like growth factor-I
  • Neuroprotection
  • Phosphorylation
  • Protein kinase B
  • Signal transduction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alajajian, B. B., Fletcher, L., Isgor, E., Jimenez, D. F., & Digicaylioglu, M. (2009). IGF-I regulated phosphorylation and translocation of PDK-1 in neurons. NeuroReport, 20(6), 579-583. https://doi.org/10.1097/WNR.0b013e328329a41a

IGF-I regulated phosphorylation and translocation of PDK-1 in neurons. / Alajajian, Betty B.; Fletcher, Lauren; Isgor, Elif; Jimenez, David F.; Digicaylioglu, Murat.

In: NeuroReport, Vol. 20, No. 6, 22.04.2009, p. 579-583.

Research output: Contribution to journalArticle

Alajajian, BB, Fletcher, L, Isgor, E, Jimenez, DF & Digicaylioglu, M 2009, 'IGF-I regulated phosphorylation and translocation of PDK-1 in neurons', NeuroReport, vol. 20, no. 6, pp. 579-583. https://doi.org/10.1097/WNR.0b013e328329a41a
Alajajian BB, Fletcher L, Isgor E, Jimenez DF, Digicaylioglu M. IGF-I regulated phosphorylation and translocation of PDK-1 in neurons. NeuroReport. 2009 Apr 22;20(6):579-583. https://doi.org/10.1097/WNR.0b013e328329a41a
Alajajian, Betty B. ; Fletcher, Lauren ; Isgor, Elif ; Jimenez, David F. ; Digicaylioglu, Murat. / IGF-I regulated phosphorylation and translocation of PDK-1 in neurons. In: NeuroReport. 2009 ; Vol. 20, No. 6. pp. 579-583.
@article{86f91bfae31540c18142b6ef0c92943b,
title = "IGF-I regulated phosphorylation and translocation of PDK-1 in neurons",
abstract = "3′-Phosphoinositide-dependent protein kinase-1 (PDK-1) is a crucial serine/threonine kinase in the insulin-like growth factor-I (IGF-I)/AKT signaling pathway, but its function and localization in the nervous system has not been fully characterized. In this study, we compared the localization of PDK-1 in adult neurons and non-neuronal PC-3 cells. We showed that PC-3 cells expressed phosphorylated and nonphosphorylated PDK-1 in the cytoplasm and nucleoplasm. In contrast, neuronal PDK-1 was located in the nucleoplasm and the phosphorylated form was located along the perinuclear region. Furthermore, we found that IGF-I transiently increased phosphorylation of neuronal PDK-1, resulting in its translocation to other cellular compartments. Our findings suggest that IGF-I may regulate neuronal PDK-1 differently than in non-neuronal cells, which may indicate a novel role for PDK-1 in IGF-I-mediated neuroprotective signaling.",
keywords = "3'-Phosphoinositide-dependent protein kinase-1, AGC kinase, AKT, Apoptosis, Insulin-like growth factor-I, Neuroprotection, Phosphorylation, Protein kinase B, Signal transduction",
author = "Alajajian, {Betty B.} and Lauren Fletcher and Elif Isgor and Jimenez, {David F.} and Murat Digicaylioglu",
year = "2009",
month = "4",
day = "22",
doi = "10.1097/WNR.0b013e328329a41a",
language = "English (US)",
volume = "20",
pages = "579--583",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - IGF-I regulated phosphorylation and translocation of PDK-1 in neurons

AU - Alajajian, Betty B.

AU - Fletcher, Lauren

AU - Isgor, Elif

AU - Jimenez, David F.

AU - Digicaylioglu, Murat

PY - 2009/4/22

Y1 - 2009/4/22

N2 - 3′-Phosphoinositide-dependent protein kinase-1 (PDK-1) is a crucial serine/threonine kinase in the insulin-like growth factor-I (IGF-I)/AKT signaling pathway, but its function and localization in the nervous system has not been fully characterized. In this study, we compared the localization of PDK-1 in adult neurons and non-neuronal PC-3 cells. We showed that PC-3 cells expressed phosphorylated and nonphosphorylated PDK-1 in the cytoplasm and nucleoplasm. In contrast, neuronal PDK-1 was located in the nucleoplasm and the phosphorylated form was located along the perinuclear region. Furthermore, we found that IGF-I transiently increased phosphorylation of neuronal PDK-1, resulting in its translocation to other cellular compartments. Our findings suggest that IGF-I may regulate neuronal PDK-1 differently than in non-neuronal cells, which may indicate a novel role for PDK-1 in IGF-I-mediated neuroprotective signaling.

AB - 3′-Phosphoinositide-dependent protein kinase-1 (PDK-1) is a crucial serine/threonine kinase in the insulin-like growth factor-I (IGF-I)/AKT signaling pathway, but its function and localization in the nervous system has not been fully characterized. In this study, we compared the localization of PDK-1 in adult neurons and non-neuronal PC-3 cells. We showed that PC-3 cells expressed phosphorylated and nonphosphorylated PDK-1 in the cytoplasm and nucleoplasm. In contrast, neuronal PDK-1 was located in the nucleoplasm and the phosphorylated form was located along the perinuclear region. Furthermore, we found that IGF-I transiently increased phosphorylation of neuronal PDK-1, resulting in its translocation to other cellular compartments. Our findings suggest that IGF-I may regulate neuronal PDK-1 differently than in non-neuronal cells, which may indicate a novel role for PDK-1 in IGF-I-mediated neuroprotective signaling.

KW - 3'-Phosphoinositide-dependent protein kinase-1

KW - AGC kinase

KW - AKT

KW - Apoptosis

KW - Insulin-like growth factor-I

KW - Neuroprotection

KW - Phosphorylation

KW - Protein kinase B

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=67049097602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67049097602&partnerID=8YFLogxK

U2 - 10.1097/WNR.0b013e328329a41a

DO - 10.1097/WNR.0b013e328329a41a

M3 - Article

C2 - 19276999

AN - SCOPUS:67049097602

VL - 20

SP - 579

EP - 583

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 6

ER -