Abstract
3′-Phosphoinositide-dependent protein kinase-1 (PDK-1) is a crucial serine/threonine kinase in the insulin-like growth factor-I (IGF-I)/AKT signaling pathway, but its function and localization in the nervous system has not been fully characterized. In this study, we compared the localization of PDK-1 in adult neurons and non-neuronal PC-3 cells. We showed that PC-3 cells expressed phosphorylated and nonphosphorylated PDK-1 in the cytoplasm and nucleoplasm. In contrast, neuronal PDK-1 was located in the nucleoplasm and the phosphorylated form was located along the perinuclear region. Furthermore, we found that IGF-I transiently increased phosphorylation of neuronal PDK-1, resulting in its translocation to other cellular compartments. Our findings suggest that IGF-I may regulate neuronal PDK-1 differently than in non-neuronal cells, which may indicate a novel role for PDK-1 in IGF-I-mediated neuroprotective signaling.
Original language | English (US) |
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Pages (from-to) | 579-583 |
Number of pages | 5 |
Journal | NeuroReport |
Volume | 20 |
Issue number | 6 |
DOIs | |
State | Published - Apr 22 2009 |
Keywords
- 3'-Phosphoinositide-dependent protein kinase-1
- AGC kinase
- AKT
- Apoptosis
- Insulin-like growth factor-I
- Neuroprotection
- Phosphorylation
- Protein kinase B
- Signal transduction
ASJC Scopus subject areas
- Neuroscience(all)