Idiotype network components are involved in the murine immune response to simian virus 40 large tumor antigen

Raymond L. Mernaugh, Michael H. Shearer, Robert K. Bright, Robert E. Lanford, Ronald C. Kennedy

    Research output: Contribution to journalArticlepeer-review

    9 Scopus citations

    Abstract

    Baculovirus-derived recombinant simian virus 40 (SV40) large tumor antigen (SV40 T-Ag), a monoclonal antibody specific for SV40 T-Ag (Ab-1 preparation), and a monoclonal anti-idiotypic antibody (anti-Id), designated 58D, were used to analyze the humoral immune response of Balb/c mice either immunized with recombinant SV40 T-Ag or challenged with SV40-transformed cells. Inhibition assays indicated that antibodies from mice immunized with SV40 T-Ag and from those bearing SV40 tumor inhibited the SV40 T-Ag/Ab-1 reaction. These data suggested that the antibody response in immunized or tumorchallenged mice recognized similar epitope(s) on SV40 T-Ag to that detected by the monoclonal Ab-1. These anti-(SV40 T-Ag) response antibodies also inhibited the Ab-1/anti-Id reaction and recognized the anti-Id in direct binding assays. Together, these data indicate that murine anti-(SV40 T-Ag) responses shared an idiotope with a monoclonal anti-(SV40 T-Ag) Ab-1 preparation. This idiotope, which is recognized by the monoclonal anti-Id preparation, 58D, appears to be involved in the humoral immune response to SV40 T-Ag in both SV40-T-Ag-immunized and tumor-bearing mice. The monoclonal anti-Id preparation may represent a focal point for manipulating the humoral immune response to tumors induced by SV40-transformed cells.

    Original languageEnglish (US)
    Pages (from-to)113-118
    Number of pages6
    JournalCancer Immunology Immunotherapy
    Volume35
    Issue number2
    DOIs
    StatePublished - Mar 1 1992

    Keywords

    • Idiotype components
    • Simian virus 40

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Oncology
    • Cancer Research

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