Identifying influenza viruses with resequencing microarrays

Zheng Wang, Luke T. Daum, Gary J. Vora, David Metzgar, Elizabeth A Walter, Linda C. Canas, Anthony P. Malanoski, Baochuan Lin, David A. Stenger

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Identification of genetic variations of influenza viruses is essential for epidemic and pandemic outbreak surveillance and determination of vaccine strain selection. In this study, we combined a random amplification strategy with high-density resequencing microarray technology to demonstrate simultaneous detection and sequence-based typing of 25 geographically distributed human influenza virus strains collected in 2004 and 2005. In addition to identification, this method provided primary sequence information, which suggested that distinct lineages of influenza viruses co-circulated during the 2004-2005 season, and simultaneously identified and typed all component strains of the trivalent FluMist intranasal vaccine. The results demonstrate a novel, timely, and unbiased method for the molecular epidemiologic surveillance of influenza viruses.

Original languageEnglish (US)
Pages (from-to)638-646
Number of pages9
JournalEmerging Infectious Diseases
Volume12
Issue number4
StatePublished - Apr 2006
Externally publishedYes

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Orthomyxoviridae
Vaccines
Epidemiological Monitoring
Influenza Vaccines
Pandemics
Human Influenza
Disease Outbreaks
Technology

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Wang, Z., Daum, L. T., Vora, G. J., Metzgar, D., Walter, E. A., Canas, L. C., ... Stenger, D. A. (2006). Identifying influenza viruses with resequencing microarrays. Emerging Infectious Diseases, 12(4), 638-646.

Identifying influenza viruses with resequencing microarrays. / Wang, Zheng; Daum, Luke T.; Vora, Gary J.; Metzgar, David; Walter, Elizabeth A; Canas, Linda C.; Malanoski, Anthony P.; Lin, Baochuan; Stenger, David A.

In: Emerging Infectious Diseases, Vol. 12, No. 4, 04.2006, p. 638-646.

Research output: Contribution to journalArticle

Wang, Z, Daum, LT, Vora, GJ, Metzgar, D, Walter, EA, Canas, LC, Malanoski, AP, Lin, B & Stenger, DA 2006, 'Identifying influenza viruses with resequencing microarrays', Emerging Infectious Diseases, vol. 12, no. 4, pp. 638-646.
Wang Z, Daum LT, Vora GJ, Metzgar D, Walter EA, Canas LC et al. Identifying influenza viruses with resequencing microarrays. Emerging Infectious Diseases. 2006 Apr;12(4):638-646.
Wang, Zheng ; Daum, Luke T. ; Vora, Gary J. ; Metzgar, David ; Walter, Elizabeth A ; Canas, Linda C. ; Malanoski, Anthony P. ; Lin, Baochuan ; Stenger, David A. / Identifying influenza viruses with resequencing microarrays. In: Emerging Infectious Diseases. 2006 ; Vol. 12, No. 4. pp. 638-646.
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