Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival

Aaron M. Elliott, Maria P. de Miguel, Vivienne I. Rebel, Peter J. Donovan

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Primordial germ cells (PGCs) are the embryonic precursors of the adult gametes. Although restricted in developmental potency, PGCs express many of the same molecular markers as pluripotent embryonic stem (ES) cells and can give rise to embryonal carcinoma (EC) cells, the stem cells of testicular tumors, in vivo. Likewise, when exposed to specific growth factors in vitro PGCs can be converted into pluripotent embryonic germ (EG) cells. Here, we propose that genes differentially expressed between PGCs and ES cells are good candidates for regulating germline development. To identify genes important in regulating germ cell development and mammalian fertility, we performed suppression subtraction hybridization (SSH) between PGCs and ES cells whole gene set. Using this method, we identified the transcriptional coactivator/histone acetyltransferase CREB-binding protein (CBP) as being highly expressed in PGCs compared to ES cells. To elucidate the function of CBP in PGCs, we generated mice with a PGC-specific deletion of CBP. Loss of CBP in PGCs leads to increased apoptosis and subsequent reduction in PGC numbers. These data indicate an essential role for CBP in maintaining normal germ cell development.

Original languageEnglish (US)
Pages (from-to)347-358
Number of pages12
JournalDevelopmental Biology
Volume311
Issue number2
DOIs
StatePublished - Nov 15 2007

Keywords

  • Apoptosis
  • CREB-binding protein (CBP)
  • Developmental potency
  • Embryonal carcinoma (EC) cell
  • Embryonic germ (EG) cell
  • Embryonic stem (ES) cell
  • Fertility
  • Germ cell
  • Primordial germ cell (PGC)
  • Suppression subtraction hybridization (SSH)
  • Survival

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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