TY - JOUR
T1 - Identification of viral infections in the prostate and evaluation of their association with cancer
AU - Martinez-Fierro, Margarita L.
AU - Leach, Robin J.
AU - Gomez-Guerra, Lauro S.
AU - Garza-Guajardo, Raquel
AU - Johnson-Pais, Teresa
AU - Beuten, Joke
AU - Morales-Rodriguez, Idelma B.
AU - Hernandez-Ordoñez, Mario A.
AU - Calderon-Cardenas, German
AU - Ortiz-Lopez, Rocio
AU - Rivas-Estilla, Ana M.
AU - Ancer-Rodriguez, Jesus
AU - Rojas-Martinez, Augusto
N1 - Funding Information:
We thank all the study participants. Authors appreciate contributions from Dr. Ian M. Thompson and Dr. Dean Troyer for their scientific advice and to Mrs. Betsy A. Higgins, and Mrs. Rosario Mendez-Meza for their assistance and technical support. This work was supported by CONACyT-SALUD Grant 14085-2005 and PAICYT/UANL Grant SA1445-06 to ARM.
PY - 2010/6/24
Y1 - 2010/6/24
N2 - Background: Several viruses with known oncogenic potential infect prostate tissue, among these are the polyomaviruses BKV, JCV, and SV40; human papillomaviruses (HPVs), and human cytomegalovirus (HCMV) infections. Recently, the Xenotropic Murine Leukemia Virus-related gammaretrovirus (XMRV) was identified in prostate tissue with a high prevalence observed in prostate cancer (PC) patients homozygous for the glutamine variant of the RNASEL protein (462Q/Q). Association studies with the R462Q allele and non-XMRV viruses have not been reported. We assessed associations between prostate cancer, prostate viral infections, and the RNASEL 462Q allele in Mexican cancer patients and controls.Methods: 130 subjects (55 prostate cancer cases and 75 controls) were enrolled in the study. DNA and RNA isolated from prostate tissues were screened for the presence of viral genomes. Genotyping of the RNASEL R462Q variant was performed by Taqman method.Results: R/R, R/Q, and Q/Q frequencies for R462Q were 0.62, 0.38, and 0.0 for PC cases and 0.69, 0.24, and 0.07 for controls, respectively. HPV sequences were detected in 11 (20.0%) cases and 4 (5.3%) controls. XMRV and HCMV infections were detected in one and six control samples, respectively. The risk of PC was significantly increased (Odds Ratio = 3.98; 95% CI: 1.17-13.56, p = 0.027) by infection of the prostatic tissue with HPV. BKV, JCV, and SV40 sequences were not detected in any of the tissue samples examined.Conclusions: We report a positive association between PC and HPV infection. The 462Q/Q RNASEL genotype was not represented in our PC cases; thus, its interaction with prostate viral infections and cancer could not be evaluated.
AB - Background: Several viruses with known oncogenic potential infect prostate tissue, among these are the polyomaviruses BKV, JCV, and SV40; human papillomaviruses (HPVs), and human cytomegalovirus (HCMV) infections. Recently, the Xenotropic Murine Leukemia Virus-related gammaretrovirus (XMRV) was identified in prostate tissue with a high prevalence observed in prostate cancer (PC) patients homozygous for the glutamine variant of the RNASEL protein (462Q/Q). Association studies with the R462Q allele and non-XMRV viruses have not been reported. We assessed associations between prostate cancer, prostate viral infections, and the RNASEL 462Q allele in Mexican cancer patients and controls.Methods: 130 subjects (55 prostate cancer cases and 75 controls) were enrolled in the study. DNA and RNA isolated from prostate tissues were screened for the presence of viral genomes. Genotyping of the RNASEL R462Q variant was performed by Taqman method.Results: R/R, R/Q, and Q/Q frequencies for R462Q were 0.62, 0.38, and 0.0 for PC cases and 0.69, 0.24, and 0.07 for controls, respectively. HPV sequences were detected in 11 (20.0%) cases and 4 (5.3%) controls. XMRV and HCMV infections were detected in one and six control samples, respectively. The risk of PC was significantly increased (Odds Ratio = 3.98; 95% CI: 1.17-13.56, p = 0.027) by infection of the prostatic tissue with HPV. BKV, JCV, and SV40 sequences were not detected in any of the tissue samples examined.Conclusions: We report a positive association between PC and HPV infection. The 462Q/Q RNASEL genotype was not represented in our PC cases; thus, its interaction with prostate viral infections and cancer could not be evaluated.
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U2 - 10.1186/1471-2407-10-326
DO - 10.1186/1471-2407-10-326
M3 - Article
C2 - 20576103
AN - SCOPUS:77955355119
VL - 10
JO - BMC Cancer
JF - BMC Cancer
SN - 1471-2407
M1 - 326
ER -