Identification of the phosphorylated β-tubulin isotype in differentiated neuroblastoma cells

Richard F. Ludueña; Hans Peter Zimmermann; Melvyn Little

doi:10.1016/0014-5793(88)80658-6

Identification of the phosphorylated β-tubulin isotype in differentiated neuroblastoma cells

Richard F. Ludueña, Hans Peter Zimmermann, Melvyn Little

Biochemistry & Structural Biology

Research output: Contribution to journal › Article

56 Scopus citations

Abstract

The tubulin molecule consists of an α- and a β-subunit, each of which exists in several isotypic forms. It has been previously shown that one of the isotypes of neuroblastoma β-tubulin is phosphorylated at a serine residue in vivo [(1985) J. Cell Biol. 100, 764-774]. Here we identify the phosphorylated isotype as β₂ (type III). Moreover, the large size of the phosphorylated tryptic peptide and sequence comparisons of vertebrate β-tubulins suggest that one of the two serines in positions 444 and 446 is the phosphorylated residue. Our results raise the possibility that β₂-tubulin differs functionally from the other β-tubulin isotypes.

Original language	English (US)
Pages (from-to)	142-146
Number of pages	5
Journal	FEBS Letters
Volume	230
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(88)80658-6
State	Published - Mar 28 1988

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Keywords

Neuroblastoma tubulin
Tubulin isotype
Tubulin phosphorylation
β-Tubulin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Cite this

Ludueña, R. F., Zimmermann, H. P., & Little, M. (1988). Identification of the phosphorylated β-tubulin isotype in differentiated neuroblastoma cells. FEBS Letters, 230(1-2), 142-146. https://doi.org/10.1016/0014-5793(88)80658-6

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10.1016/0014-5793(88)80658-6