TY - JOUR
T1 - Identification of regions of pRZ2 which have delayed elution behavior on RPC-5 column chromatography.
AU - Patient, R. K.
AU - Hardies, S. C.
AU - Wells, R. D.
N1 - Copyright:
Medline is the source for the citation and abstract of this record.
PY - 1979/6/25
Y1 - 1979/6/25
N2 - DNA restriction fragments generally elute from RPC-5 in order of their size. However, some fragments elute later than predicted. Chromatographic studies were performed on five different restriction digests (Hae III, Hha I, Alu I, Taq I, and Hae III + HindII) of pRZ2 DNA in an effort to localize the regions which have the delayed properties. Also, the magnitude of the delay was quantitated in each case. Most of the delayed fragments were localized in one major (931 bp) and one minor (approximately 210 bp) region of the genome. The fragments exhibiting a greater extent of delay were in the major region. The results described herein and in the following paper show that, in most cases, this effect can be explained by the base composition, or sequence of the fragments, or both.
AB - DNA restriction fragments generally elute from RPC-5 in order of their size. However, some fragments elute later than predicted. Chromatographic studies were performed on five different restriction digests (Hae III, Hha I, Alu I, Taq I, and Hae III + HindII) of pRZ2 DNA in an effort to localize the regions which have the delayed properties. Also, the magnitude of the delay was quantitated in each case. Most of the delayed fragments were localized in one major (931 bp) and one minor (approximately 210 bp) region of the genome. The fragments exhibiting a greater extent of delay were in the major region. The results described herein and in the following paper show that, in most cases, this effect can be explained by the base composition, or sequence of the fragments, or both.
UR - http://www.scopus.com/inward/record.url?scp=0018801056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018801056&partnerID=8YFLogxK
M3 - Article
C2 - 376512
AN - SCOPUS:0018801056
VL - 254
SP - 5542
EP - 5547
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 12
ER -