Identification of regions of pRZ2 which have delayed elution behavior on RPC-5 column chromatography.

R. K. Patient, S. C. Hardies, R. D. Wells

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

DNA restriction fragments generally elute from RPC-5 in order of their size. However, some fragments elute later than predicted. Chromatographic studies were performed on five different restriction digests (Hae III, Hha I, Alu I, Taq I, and Hae III + HindII) of pRZ2 DNA in an effort to localize the regions which have the delayed properties. Also, the magnitude of the delay was quantitated in each case. Most of the delayed fragments were localized in one major (931 bp) and one minor (approximately 210 bp) region of the genome. The fragments exhibiting a greater extent of delay were in the major region. The results described herein and in the following paper show that, in most cases, this effect can be explained by the base composition, or sequence of the fragments, or both.

Original languageEnglish (US)
Pages (from-to)5542-5547
Number of pages6
JournalJournal of Biological Chemistry
Volume254
Issue number12
StatePublished - Jun 25 1979

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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