Identification of Rat Urinary Proteins by Zone and Immunoelectrophoresis

Arun K. Roy, Otto W. Neuhaus

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30 Scopus citations


Separation and identification of male rat urinary proteins have been accomplished by zone electrophoresis on paper, in dilute agar and starch-gel media and by immunoelectrophoresis. Approximately 15 individual proteins have been delineated of which 7 are derived from serum while the remainder appear to be unique to the urine. The serum proteins are albumin, α1 of Darcy, α1-S3.5 glycoprotein, haptoglobin, “pi” protein, transferrin and γ globulin. Those associated only with urine are 2 prealbumins, a protein migrating as does albumin, an α1 globulin, 2 α2, 1 β, and a γ globulin. One of the α2 globulins, α2u, is quantitatively the major component of the urinary proteins and appears to be glycoprotein in nature. ADDENDUM. After preparation and submission of this manuscript, a report by Bao-Linh et al (20) appeared describing the existence of 5 proteins unique to rat urine. The major urinary component was also shown to be an α2 globulin.

Original languageEnglish (US)
Pages (from-to)894-899
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Issue number3
StatePublished - Mar 1966
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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