TY - JOUR
T1 - Identification of Rat Urinary Proteins by Zone and Immunoelectrophoresis
AU - Roy, Arun K.
AU - Neuhaus, Otto W.
N1 - Funding Information:
Studies reported here presented in part at Federation of Am. Soc. for Exp. Biol., April 16, 1965(1). This investigation was supported by a grant from USPHS, AM-06705.
PY - 1966/3
Y1 - 1966/3
N2 - Separation and identification of male rat urinary proteins have been accomplished by zone electrophoresis on paper, in dilute agar and starch-gel media and by immunoelectrophoresis. Approximately 15 individual proteins have been delineated of which 7 are derived from serum while the remainder appear to be unique to the urine. The serum proteins are albumin, α1 of Darcy, α1-S3.5 glycoprotein, haptoglobin, “pi” protein, transferrin and γ globulin. Those associated only with urine are 2 prealbumins, a protein migrating as does albumin, an α1 globulin, 2 α2, 1 β, and a γ globulin. One of the α2 globulins, α2u, is quantitatively the major component of the urinary proteins and appears to be glycoprotein in nature. ADDENDUM. After preparation and submission of this manuscript, a report by Bao-Linh et al (20) appeared describing the existence of 5 proteins unique to rat urine. The major urinary component was also shown to be an α2 globulin.
AB - Separation and identification of male rat urinary proteins have been accomplished by zone electrophoresis on paper, in dilute agar and starch-gel media and by immunoelectrophoresis. Approximately 15 individual proteins have been delineated of which 7 are derived from serum while the remainder appear to be unique to the urine. The serum proteins are albumin, α1 of Darcy, α1-S3.5 glycoprotein, haptoglobin, “pi” protein, transferrin and γ globulin. Those associated only with urine are 2 prealbumins, a protein migrating as does albumin, an α1 globulin, 2 α2, 1 β, and a γ globulin. One of the α2 globulins, α2u, is quantitatively the major component of the urinary proteins and appears to be glycoprotein in nature. ADDENDUM. After preparation and submission of this manuscript, a report by Bao-Linh et al (20) appeared describing the existence of 5 proteins unique to rat urine. The major urinary component was also shown to be an α2 globulin.
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U2 - 10.3181/00379727-121-30917
DO - 10.3181/00379727-121-30917
M3 - Article
C2 - 4160706
AN - SCOPUS:0013887960
SN - 1535-3702
VL - 121
SP - 894
EP - 899
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
IS - 3
ER -