Identification of proteins crosslinked to RNA in 40S ribosomal subunits of Saccharomyces cerevisiae

Rui Hua Xiang, John C. Lee

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

RNA-protein crosslinks were introduced into the 40S ribosomal subunits from Saccharomyces cerevisiae by mild UV treatment. Proteins crosslinked to the 18S rRNA molecule were separated from free proteins by repeated extraction of the treated subunits and centrifugation in glycerol gradients. After digestion with RNase to remove the RNA molecules, proteins were radio-labeled with 125I and identified by electrophoresis on two-dimensional polyacrylamide gels with carrier total 40S ribosomal proteins and autoradiography. Proteins S2, S7, S13, S14, S17/22/27, and S18 were linked to the 18S rRNA. A shorter period of irradiation resulted in crosslinking of S2 and S17/22/27 only. Several of these proteins were previously demonstrated to be present in ribosomal core particles or early assembled proteins.

Original languageEnglish (US)
Pages (from-to)1201-1204
Number of pages4
JournalBiochimie
Volume71
Issue number11-12
DOIs
StatePublished - Jan 1 1989

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Keywords

  • crosslinking
  • ribosome
  • yeast

ASJC Scopus subject areas

  • Biochemistry

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