Identification of platelet activating factor isolated from rabbit basophils as acetyl glyceryl ether phosphorylcholine

D. J. Hanahan; C. A. Demopoulos; J. Liehr; R. N. Pinckard

Identification of platelet activating factor isolated from rabbit basophils as acetyl glyceryl ether phosphorylcholine

D. J. Hanahan, C. A. Demopoulos, J. Liehr, R. N. Pinckard

Pathology

Research output: Contribution to journal › Article › peer-review

289 Scopus citations

Abstract

Platelet activating factor obtained from antigen-stimulated rabbit buffy coat leukocytes containing 10% IgE-sensitized basophils was shown by gas-liquid chromatographic and mass spectrometric analysis of products after acetolysis and by base and acid hydrolysis to be acetyl-alkylglyceryl phosphorylcholine. The alkyl side chains were composed of octadecyl (>90%) and hexadecyl (<10%) residues.

Original language	English (US)
Pages (from-to)	5514-5516
Number of pages	3
Journal	Journal of Biological Chemistry
Volume	255
Issue number	12
State	Published - 1980

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Identification of platelet activating factor isolated from rabbit basophils as acetyl glyceryl ether phosphorylcholine",

abstract = "Platelet activating factor obtained from antigen-stimulated rabbit buffy coat leukocytes containing 10% IgE-sensitized basophils was shown by gas-liquid chromatographic and mass spectrometric analysis of products after acetolysis and by base and acid hydrolysis to be acetyl-alkylglyceryl phosphorylcholine. The alkyl side chains were composed of octadecyl (>90%) and hexadecyl (<10%) residues.",

author = "Hanahan, {D. J.} and Demopoulos, {C. A.} and J. Liehr and Pinckard, {R. N.}",

year = "1980",

language = "English (US)",

volume = "255",

pages = "5514--5516",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "12",

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AU - Hanahan, D. J.

AU - Demopoulos, C. A.

AU - Liehr, J.

AU - Pinckard, R. N.

PY - 1980

Y1 - 1980

N2 - Platelet activating factor obtained from antigen-stimulated rabbit buffy coat leukocytes containing 10% IgE-sensitized basophils was shown by gas-liquid chromatographic and mass spectrometric analysis of products after acetolysis and by base and acid hydrolysis to be acetyl-alkylglyceryl phosphorylcholine. The alkyl side chains were composed of octadecyl (>90%) and hexadecyl (<10%) residues.

AB - Platelet activating factor obtained from antigen-stimulated rabbit buffy coat leukocytes containing 10% IgE-sensitized basophils was shown by gas-liquid chromatographic and mass spectrometric analysis of products after acetolysis and by base and acid hydrolysis to be acetyl-alkylglyceryl phosphorylcholine. The alkyl side chains were composed of octadecyl (>90%) and hexadecyl (<10%) residues.

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VL - 255

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