Identification of Native Flavin Adducts Fusarium oxysporum Using Accurate Mass Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Ricky D. Edmondson, Giovanni Gadda, Paul F. Fitzpatrick, David H. Russell

Research output: Contribution to journalArticle

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Abstract

Accurate mass matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry is used to determine the identity of a naturally occurring substituted flavin adenine dinucleotide (FAD) of nitroalkane oxidase isolated from Fusarium oxysporum. Accurate mass measurements (1-2 ppm) combined with an elemental composition search identifies the unknown FAD substituent as nitrobutane. The use of internal and external calibration in accurate mass MALDI-TOF is discussed.

Original languageEnglish (US)
Pages (from-to)2862-2865
Number of pages4
JournalAnalytical Chemistry
Volume69
Issue number14
StatePublished - Jul 15 1997

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ASJC Scopus subject areas

  • Analytical Chemistry

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