Identification of human and mouse homologs of the MC51L-53L-54L family of secreted glycoproteins encoded by the molluscum contagiosum poxvirus

Yan Xiang, Bernard Moss

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Molluscum contagiosum virus (MCV) is a human poxvirus that produces small, benign skin tumors primarily in young children and encodes proteins that modulate the host immune response. The MC51L, MC53L, and MC54L open reading frames of MCV have significant amino acid sequence similarities and related proteins are encoded by other poxviruses. These three MCV genes were individually expressed in mammalian cells as glycosylated and secreted proteins. A database search detected partial sequences of homologous human and mouse cDNAs; determination of the complete sequences confirmed the homology and indicated potential signal peptides and N-glycosylation sites as well as a pattern of cysteines that are conserved in the viral proteins. The human gene, which was mapped to chromosome 11q13, was highly expressed in spleen and lymph nodes, suggesting an immune modulatory role. The latter finding is consistent with a recent report (Novick et al., 1999, Immunity 10, 127-136) that the human protein binds IL-18, suggesting an anti-inflammatory role for the poxvirus homologs.

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalVirology
Volume257
Issue number2
DOIs
StatePublished - May 10 1999
Externally publishedYes

Fingerprint

Molluscum Contagiosum
Poxviridae
Molluscum contagiosum virus
Forensic Anthropology
Glycoproteins
Sequence Homology
Proteins
Interleukin-18
Viral Proteins
Protein Sorting Signals
Glycosylation
Open Reading Frames
Genes
Cysteine
Amino Acid Sequence
Immunity
Anti-Inflammatory Agents
Spleen
Complementary DNA
Chromosomes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Identification of human and mouse homologs of the MC51L-53L-54L family of secreted glycoproteins encoded by the molluscum contagiosum poxvirus. / Xiang, Yan; Moss, Bernard.

In: Virology, Vol. 257, No. 2, 10.05.1999, p. 297-302.

Research output: Contribution to journalArticle

@article{06f892b3522c43b6bc118d09cb1dbedf,
title = "Identification of human and mouse homologs of the MC51L-53L-54L family of secreted glycoproteins encoded by the molluscum contagiosum poxvirus",
abstract = "Molluscum contagiosum virus (MCV) is a human poxvirus that produces small, benign skin tumors primarily in young children and encodes proteins that modulate the host immune response. The MC51L, MC53L, and MC54L open reading frames of MCV have significant amino acid sequence similarities and related proteins are encoded by other poxviruses. These three MCV genes were individually expressed in mammalian cells as glycosylated and secreted proteins. A database search detected partial sequences of homologous human and mouse cDNAs; determination of the complete sequences confirmed the homology and indicated potential signal peptides and N-glycosylation sites as well as a pattern of cysteines that are conserved in the viral proteins. The human gene, which was mapped to chromosome 11q13, was highly expressed in spleen and lymph nodes, suggesting an immune modulatory role. The latter finding is consistent with a recent report (Novick et al., 1999, Immunity 10, 127-136) that the human protein binds IL-18, suggesting an anti-inflammatory role for the poxvirus homologs.",
author = "Yan Xiang and Bernard Moss",
year = "1999",
month = "5",
day = "10",
doi = "10.1006/viro.1999.9676",
language = "English (US)",
volume = "257",
pages = "297--302",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Identification of human and mouse homologs of the MC51L-53L-54L family of secreted glycoproteins encoded by the molluscum contagiosum poxvirus

AU - Xiang, Yan

AU - Moss, Bernard

PY - 1999/5/10

Y1 - 1999/5/10

N2 - Molluscum contagiosum virus (MCV) is a human poxvirus that produces small, benign skin tumors primarily in young children and encodes proteins that modulate the host immune response. The MC51L, MC53L, and MC54L open reading frames of MCV have significant amino acid sequence similarities and related proteins are encoded by other poxviruses. These three MCV genes were individually expressed in mammalian cells as glycosylated and secreted proteins. A database search detected partial sequences of homologous human and mouse cDNAs; determination of the complete sequences confirmed the homology and indicated potential signal peptides and N-glycosylation sites as well as a pattern of cysteines that are conserved in the viral proteins. The human gene, which was mapped to chromosome 11q13, was highly expressed in spleen and lymph nodes, suggesting an immune modulatory role. The latter finding is consistent with a recent report (Novick et al., 1999, Immunity 10, 127-136) that the human protein binds IL-18, suggesting an anti-inflammatory role for the poxvirus homologs.

AB - Molluscum contagiosum virus (MCV) is a human poxvirus that produces small, benign skin tumors primarily in young children and encodes proteins that modulate the host immune response. The MC51L, MC53L, and MC54L open reading frames of MCV have significant amino acid sequence similarities and related proteins are encoded by other poxviruses. These three MCV genes were individually expressed in mammalian cells as glycosylated and secreted proteins. A database search detected partial sequences of homologous human and mouse cDNAs; determination of the complete sequences confirmed the homology and indicated potential signal peptides and N-glycosylation sites as well as a pattern of cysteines that are conserved in the viral proteins. The human gene, which was mapped to chromosome 11q13, was highly expressed in spleen and lymph nodes, suggesting an immune modulatory role. The latter finding is consistent with a recent report (Novick et al., 1999, Immunity 10, 127-136) that the human protein binds IL-18, suggesting an anti-inflammatory role for the poxvirus homologs.

UR - http://www.scopus.com/inward/record.url?scp=0033541964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033541964&partnerID=8YFLogxK

U2 - 10.1006/viro.1999.9676

DO - 10.1006/viro.1999.9676

M3 - Article

C2 - 10329540

AN - SCOPUS:0033541964

VL - 257

SP - 297

EP - 302

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -