Identification of genes required for damage survival using a cell-based RNAi screen against the drosophila genome

Dashnamoorthy Ravi, Alexander J Bishop

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Exposure to DNA-damaging agents invokes biological responses necessary for damage recovery and cell survival. Despite the presence of intact DNA repair pathways, lack of certain other biological pathways has been shown to sensitize cells to DNA-damaging agents' exposure. It is likely that following DNA damage a complex interplay between DNA repair pathways and other biological pathways might be required to ensure cell survival. In this chapter, we describe a high-throughput method for the identification of genes essential for cell survival following DNA damage by using a cell-based assay to measure viability in combination with an RNA interference-based genome-wide screening experiment.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages9-26
Number of pages18
Volume920
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume920
ISSN (Print)10643745

Fingerprint

RNA Interference
Drosophila
Cell Survival
Genome
DNA Repair
DNA Damage
Genes
DNA
Essential Genes

Keywords

  • DNA damage
  • Drosophila
  • Genome-wide screening
  • RNAi
  • Survival

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Identification of genes required for damage survival using a cell-based RNAi screen against the drosophila genome. / Ravi, Dashnamoorthy; Bishop, Alexander J.

Methods in Molecular Biology. Vol. 920 2012. p. 9-26 (Methods in Molecular Biology; Vol. 920).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ravi, Dashnamoorthy ; Bishop, Alexander J. / Identification of genes required for damage survival using a cell-based RNAi screen against the drosophila genome. Methods in Molecular Biology. Vol. 920 2012. pp. 9-26 (Methods in Molecular Biology).
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