Identification of genes required for damage survival using a cell-based RNAi screen against the drosophila genome

Dashnamoorthy Ravi, Alexander James Roy Bishop

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Exposure to DNA-damaging agents invokes biological responses necessary for damage recovery and cell survival. Despite the presence of intact DNA repair pathways, lack of certain other biological pathways has been shown to sensitize cells to DNA-damaging agents' exposure. It is likely that following DNA damage a complex interplay between DNA repair pathways and other biological pathways might be required to ensure cell survival. In this chapter, we describe a high-throughput method for the identification of genes essential for cell survival following DNA damage by using a cell-based assay to measure viability in combination with an RNA interference-based genome-wide screening experiment.

Original languageEnglish (US)
Title of host publicationDNA Repair Protocols
PublisherHumana Press
Pages9-26
Number of pages18
ISBN (Print)9781617799976
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume920
ISSN (Print)1064-3745

Keywords

  • DNA damage
  • Drosophila
  • Genome-wide screening
  • RNAi
  • Survival

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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