Identification of araC protein on two-dimensional gels, its in vivo instability and normal level

D. Kolodrubetz, R. Schleif

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

araC protein was identified on two-dimensional O'Farrell gels (O'Farrell, 1975) as a protein electrophoresing as two spots, both of molecular weight 30,500 and pI near 7.1, but differing by about 0.1 pH unit. The two spots were seen in crude extracts from cells overproducing C protein as specified by a plasmid, by a phage, and were also seen in C protein purified to about 20% purity on the basis of biological activity. A label-chase experiment indicated that both species of araC are unstable in vivo and possess half-lives of about 60 minutes. The normal intracellular level of C protein is about 40 monomers per cell.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalJournal of Molecular Biology
Volume149
Issue number1
DOIs
StatePublished - Jun 15 1981
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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