Identification of an osteogenic protein-1 responsive element in the aggrecan promoter

Lee Chuan C. Yeh, John C. Lee

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Previous studies have demonstrated that osteogenic protein-1 (OP-1), a member of the bone morphogenetic protein (BMP) subfamily of the TGF-β superfamily, stimulates aggrecan synthesis. To investigate transcriptional regulation of the aggrecan gene by OP-1, we constructed a clone containing a 1 kb region of the 5′-upstream sequence of the mouse aggrecan gene fused to the promoter-less luciferase reporter gene in pGL2-Basic vector. A series of promoter deletion constructs were also generated. Studies of the promoter activity of these DNA constructs in transient-transfected cells revealed that deletion of a 33 bp region rendered the promoter unresponsive to OP-1, BMP-6, and CDMP-1 without affecting BMP-2 and TGF-β1 responsiveness. Thus, the expression of the mouse aggrecan gene in response to BMPs appears to be the result of a unique combination of different cis-acting elements.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume323
Issue number1
DOIs
StatePublished - Oct 8 2004

Keywords

  • Aggrecan promoter
  • BMP-7
  • Bone morphogenetic protein
  • Osteogenic protein-1
  • Transcription regulation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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