Identification of a Short Viral Transcript in Leishmania RNA Virus-infected Cells

In Kwon Chung, Timothy C. Armstrong, Jean L. Patterson

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Certain strains of Leishmania guyanensis carry persistently infecting double-stranded RNA viruses. The viral polymerase has been shown to have both transcriptase and replicase activity. To date, only full-length RNA transcription of minus and plus strands have been reported. This report describes the synthesis of a 320 nt transcript which is complementary to the 3′ end of the minus strand RNA. This plus-stranded short transcript was first detected in an in vitro polymerase assay. It was also detected in virus-infected Leishmania cells by a reverse transcription-polymerase chain reaction assay and by Northern analysis of infected Leishmania cell RNA.

Original languageEnglish (US)
Article number71066
Pages (from-to)552-556
Number of pages5
Issue number2
StatePublished - Feb 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Virology


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