Identification of a Short Viral Transcript in Leishmania RNA Virus-infected Cells

In Kwon Chung, Timothy C. Armstrong, Jean L. Patterson

    Research output: Contribution to journalArticlepeer-review

    17 Scopus citations

    Abstract

    Certain strains of Leishmania guyanensis carry persistently infecting double-stranded RNA viruses. The viral polymerase has been shown to have both transcriptase and replicase activity. To date, only full-length RNA transcription of minus and plus strands have been reported. This report describes the synthesis of a 320 nt transcript which is complementary to the 3′ end of the minus strand RNA. This plus-stranded short transcript was first detected in an in vitro polymerase assay. It was also detected in virus-infected Leishmania cells by a reverse transcription-polymerase chain reaction assay and by Northern analysis of infected Leishmania cell RNA.

    Original languageEnglish (US)
    Article number71066
    Pages (from-to)552-556
    Number of pages5
    JournalVirology
    Volume198
    Issue number2
    DOIs
    StatePublished - Feb 1 1994

    ASJC Scopus subject areas

    • Virology

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