Identification of a ribosomal frameshift in Leishmania RNA virus 1-4

Sang Eun Lee, Jae Myoung Suh, Scott Scheffter, Jean L. Patterson, In Kwon Chung

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Double-stranded Leishmania RNA virus 1-4 (LRV 1-4) has at least four open Pending frames (ORFs). The two small ORFs located near its 5' terminus, ORF1 and ORFx, could encode 34- and 60-amino acid polypeptides, respectively. ORF2 encodes an 82-kDa major capsid protein, and ORF3 encodes a 98-kDa polypeptide which contains the consensus sequence for RNA-dependent RNA polymerases of plus-strand and double-stranded RNA viruses. The complete sequence of LRV 1-4 shows that ORF2 and ORF3 overlap by 71 nucleotides, and that ORF3 lacks a potential translation initiation site, suggesting that the viral polymerase may be synthesized as a 180-kDa fusion protein with the virus capsid. In this report, we present evidence for the synthesis of a fusion protein through a ribosomal frameshift. In vitro-translation experiments and immunostudies involving antiserum against the viral capsid protein demonstrated that the overlapping 71 nucleotides of ORF2 and ORF3 are contained in a region which promotes translational frameshifting. Computer analysis of the putative frameshift region revealed a potential pseudoknot structure located within the overlapping 71 nucleotide sequence.

Original languageEnglish (US)
Pages (from-to)22-25
Number of pages4
JournalJournal of Biochemistry
Volume120
Issue number1
StatePublished - 1996
Externally publishedYes

Fingerprint

Ribosomal Frameshifting
Leishmania
RNA Viruses
Viruses
Double-Stranded RNA
Capsid Proteins
RNA
Open Reading Frames
Nucleotides
Viral Fusion Proteins
RNA Replicase
Peptides
Fusion reactions
Capsid
Consensus Sequence
Viral Proteins
Immune Sera
Amino Acids
Proteins
Experiments

Keywords

  • Capsid protein
  • Leishmania RNA virus
  • Pseudoknot structure
  • Ribosomal frameshift
  • RNA-dependent RNA polymerases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lee, S. E., Suh, J. M., Scheffter, S., Patterson, J. L., & Chung, I. K. (1996). Identification of a ribosomal frameshift in Leishmania RNA virus 1-4. Journal of Biochemistry, 120(1), 22-25.

Identification of a ribosomal frameshift in Leishmania RNA virus 1-4. / Lee, Sang Eun; Suh, Jae Myoung; Scheffter, Scott; Patterson, Jean L.; Chung, In Kwon.

In: Journal of Biochemistry, Vol. 120, No. 1, 1996, p. 22-25.

Research output: Contribution to journalArticle

Lee, SE, Suh, JM, Scheffter, S, Patterson, JL & Chung, IK 1996, 'Identification of a ribosomal frameshift in Leishmania RNA virus 1-4', Journal of Biochemistry, vol. 120, no. 1, pp. 22-25.
Lee SE, Suh JM, Scheffter S, Patterson JL, Chung IK. Identification of a ribosomal frameshift in Leishmania RNA virus 1-4. Journal of Biochemistry. 1996;120(1):22-25.
Lee, Sang Eun ; Suh, Jae Myoung ; Scheffter, Scott ; Patterson, Jean L. ; Chung, In Kwon. / Identification of a ribosomal frameshift in Leishmania RNA virus 1-4. In: Journal of Biochemistry. 1996 ; Vol. 120, No. 1. pp. 22-25.
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