Identification of a Novel Splice Variant: Human SGT1B (SUGT1B)

Yohei Niikura, Katsumi Kitagawa

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We identified a novel splice variant of human SGT1 (SUGT1), a suppressor of the G2 allele of SKP1, by analysis of 8 human EST clones whose open reading frame encoded 365 amino acids. We termed this variant SGT1B (SUGT1B) and the original SGT1A (SUGT1A). The putative SGT1B and SGT1A proteins are 91% identical, and both contain a tetratricopeptide repeat (TPR) domain, two variable regions, a CS domain, and a SGS domain. The NCBI human genome database showed that SG71B and SG71A are located on chromosome band 13q14.13. SGT1B contains an additional 33 amino acids encoded by a region between exons 5 and 6 of SG71A and lacks Ser110 of SGT1A. Immunoblotting using antibodies to N-terminal (amino acids 1-157) and C-terminal (amino acids 182-333) regions of SGT1A detected 2 bands whose sizes corresponded to those predicted for SGT1A and SGT1B. Overexpression experiments confirmed this finding. Additional immunoblot analysis demonstrated that both are highly expressed in human brain, liver, lung, and testis.

Original languageEnglish (US)
Pages (from-to)436-441
Number of pages6
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume14
Issue number6
DOIs
Publication statusPublished - Dec 1 2003

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Keywords

  • Kinetochore
  • SCF ubiquitin ligase
  • Splice variant
  • TPR (tetratricopeptide repeat) domain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

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