Identification of a novel guanine nucleotide exchange factor for the Rho GTPase

Matthew J Hart, Sanju Sharma, Nadia Elmasry, Rong Guo Qiu, Peter McCabe, Paul Polakis, Gideon Bollag

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The Rho GTPase promotes proliferation and cytoskeletal rearrangements in mammalian cells. To understand the regulation of Rho, it is important to characterize guanine nucleotide exchange factors (GEFs), which stimulate the dissociation of GDP and subsequent binding of GTP. Using Rho as an affinity ligand, we have isolated a 115-kDa protein (p115-RhoGEF) that binds specifically to the nucleotide-depleted state. A full-length cDNA encoding p115-RhoGEF was isolated, and its protein product, which exhibited sequence homology to Dbl and Lbc, catalyzed the exchange of GDP for GTP specifically on Rho and not on the Rac, Cdc42, or Ras GTPases. p115-RhoGEF is capable of regulating cell proliferation, as determined by its ability to induce the transformation of NIH 3T3 cells. Northern and Western analysis suggests that p115-RhoGEF is ubiquitously expressed. These results indicate that p115- RhoGEF may be a general regulator of Rho and its associated cellular phenotypes.

Original languageEnglish (US)
Pages (from-to)25452-25458
Number of pages7
JournalJournal of Biological Chemistry
Volume271
Issue number41
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Rho Guanine Nucleotide Exchange Factors
Guanine Nucleotide Exchange Factors
rho GTP-Binding Proteins
Nucleotides
Guanosine Triphosphate
ras Proteins
NIH 3T3 Cells
Cell proliferation
Sequence Homology
Proteins
Complementary DNA
Cells
Cell Proliferation
Ligands
Phenotype

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hart, M. J., Sharma, S., Elmasry, N., Qiu, R. G., McCabe, P., Polakis, P., & Bollag, G. (1996). Identification of a novel guanine nucleotide exchange factor for the Rho GTPase. Journal of Biological Chemistry, 271(41), 25452-25458. https://doi.org/10.1074/jbc.271.41.25452

Identification of a novel guanine nucleotide exchange factor for the Rho GTPase. / Hart, Matthew J; Sharma, Sanju; Elmasry, Nadia; Qiu, Rong Guo; McCabe, Peter; Polakis, Paul; Bollag, Gideon.

In: Journal of Biological Chemistry, Vol. 271, No. 41, 1996, p. 25452-25458.

Research output: Contribution to journalArticle

Hart, MJ, Sharma, S, Elmasry, N, Qiu, RG, McCabe, P, Polakis, P & Bollag, G 1996, 'Identification of a novel guanine nucleotide exchange factor for the Rho GTPase', Journal of Biological Chemistry, vol. 271, no. 41, pp. 25452-25458. https://doi.org/10.1074/jbc.271.41.25452
Hart, Matthew J ; Sharma, Sanju ; Elmasry, Nadia ; Qiu, Rong Guo ; McCabe, Peter ; Polakis, Paul ; Bollag, Gideon. / Identification of a novel guanine nucleotide exchange factor for the Rho GTPase. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 41. pp. 25452-25458.
@article{9fcae7733c094ab896eaccdf5da48b34,
title = "Identification of a novel guanine nucleotide exchange factor for the Rho GTPase",
abstract = "The Rho GTPase promotes proliferation and cytoskeletal rearrangements in mammalian cells. To understand the regulation of Rho, it is important to characterize guanine nucleotide exchange factors (GEFs), which stimulate the dissociation of GDP and subsequent binding of GTP. Using Rho as an affinity ligand, we have isolated a 115-kDa protein (p115-RhoGEF) that binds specifically to the nucleotide-depleted state. A full-length cDNA encoding p115-RhoGEF was isolated, and its protein product, which exhibited sequence homology to Dbl and Lbc, catalyzed the exchange of GDP for GTP specifically on Rho and not on the Rac, Cdc42, or Ras GTPases. p115-RhoGEF is capable of regulating cell proliferation, as determined by its ability to induce the transformation of NIH 3T3 cells. Northern and Western analysis suggests that p115-RhoGEF is ubiquitously expressed. These results indicate that p115- RhoGEF may be a general regulator of Rho and its associated cellular phenotypes.",
author = "Hart, {Matthew J} and Sanju Sharma and Nadia Elmasry and Qiu, {Rong Guo} and Peter McCabe and Paul Polakis and Gideon Bollag",
year = "1996",
doi = "10.1074/jbc.271.41.25452",
language = "English (US)",
volume = "271",
pages = "25452--25458",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "41",

}

TY - JOUR

T1 - Identification of a novel guanine nucleotide exchange factor for the Rho GTPase

AU - Hart, Matthew J

AU - Sharma, Sanju

AU - Elmasry, Nadia

AU - Qiu, Rong Guo

AU - McCabe, Peter

AU - Polakis, Paul

AU - Bollag, Gideon

PY - 1996

Y1 - 1996

N2 - The Rho GTPase promotes proliferation and cytoskeletal rearrangements in mammalian cells. To understand the regulation of Rho, it is important to characterize guanine nucleotide exchange factors (GEFs), which stimulate the dissociation of GDP and subsequent binding of GTP. Using Rho as an affinity ligand, we have isolated a 115-kDa protein (p115-RhoGEF) that binds specifically to the nucleotide-depleted state. A full-length cDNA encoding p115-RhoGEF was isolated, and its protein product, which exhibited sequence homology to Dbl and Lbc, catalyzed the exchange of GDP for GTP specifically on Rho and not on the Rac, Cdc42, or Ras GTPases. p115-RhoGEF is capable of regulating cell proliferation, as determined by its ability to induce the transformation of NIH 3T3 cells. Northern and Western analysis suggests that p115-RhoGEF is ubiquitously expressed. These results indicate that p115- RhoGEF may be a general regulator of Rho and its associated cellular phenotypes.

AB - The Rho GTPase promotes proliferation and cytoskeletal rearrangements in mammalian cells. To understand the regulation of Rho, it is important to characterize guanine nucleotide exchange factors (GEFs), which stimulate the dissociation of GDP and subsequent binding of GTP. Using Rho as an affinity ligand, we have isolated a 115-kDa protein (p115-RhoGEF) that binds specifically to the nucleotide-depleted state. A full-length cDNA encoding p115-RhoGEF was isolated, and its protein product, which exhibited sequence homology to Dbl and Lbc, catalyzed the exchange of GDP for GTP specifically on Rho and not on the Rac, Cdc42, or Ras GTPases. p115-RhoGEF is capable of regulating cell proliferation, as determined by its ability to induce the transformation of NIH 3T3 cells. Northern and Western analysis suggests that p115-RhoGEF is ubiquitously expressed. These results indicate that p115- RhoGEF may be a general regulator of Rho and its associated cellular phenotypes.

UR - http://www.scopus.com/inward/record.url?scp=0029794721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029794721&partnerID=8YFLogxK

U2 - 10.1074/jbc.271.41.25452

DO - 10.1074/jbc.271.41.25452

M3 - Article

C2 - 8810315

AN - SCOPUS:0029794721

VL - 271

SP - 25452

EP - 25458

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 41

ER -