Identification of a grga-euo-hrca transcriptional regulatory network in chlamydia

Wurihan Wurihan, Yi Zou, Alec M. Weber, Korri Weldon, Yehong Huang, Xiaofeng Bao, Chengsheng Zhu, Xiang Wu, Yaqun Wang, Zhao Lai, Huizhou Fan

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Chlamydia trachomatis is an obligate intracellular bacterium whose unique developmental cycle consists of an infectious elementary body and a replicative reticulate body. Progression of this developmental cycle requires temporal control of the transcriptome. In addition to the three chlamydial sigma factors (s 66, s 28, and s 54) that recognize promoter sequences of genes, chlamydial transcription factors are expected to play crucial roles in transcriptional regulation. Here, we investigate the function of GrgA, a Chlamydia-specific transcription factor, in C. trachomatis transcriptomic expression. We show that 10 to 30min of GrgA overexpression induces 13 genes, which likely comprise the direct regulon of GrgA. Significantly, s 66-dependent genes that code for two important transcription repressors are components of the direct regulon. One of these repressors is Euo, which prevents the expression of late genes during early phases. The other is HrcA, which regulates molecular chaperone expression and controls stress response. The direct regulon also includes a s 28-dependent gene that codes for the putative virulence factor PmpI. Furthermore, overexpression of GrgA leads to decreased expression of almost all tRNAs. Transcriptomic studies suggest that GrgA, Euo, and HrcA have distinct but overlapping indirect regulons. These findings, together with temporal expression patterns of grgA, euo, and hrcA, indicate that a transcriptional regulatory network of these three transcription factors plays critical roles in C. trachomatis growth and development.

Original languageEnglish (US)
Article numbere00738-21
JournalmSystems
Volume6
Issue number4
DOIs
StatePublished - Jul 2021

Keywords

  • CT504
  • CTL0766
  • Chlamydia
  • Euo
  • GrgA
  • HrcA
  • Transcription factors
  • Transcriptional regulatory network

ASJC Scopus subject areas

  • Microbiology
  • Physiology
  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Molecular Biology
  • Genetics
  • Computer Science Applications

Fingerprint

Dive into the research topics of 'Identification of a grga-euo-hrca transcriptional regulatory network in chlamydia'. Together they form a unique fingerprint.

Cite this