Identification of a DNA element that can enhance p53-mediated transactivation

Yuzuru Shiio, Tadashi Yamamoto, Nobuo Yamaguchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Recent studies have demonstrated that p53 binds to specific DNA sequences and the consensus sequence for the p53 binding site has been proposed. The p53 binding site has also been shown to act as positive p53-responsive element. Here we report the identification of a GC3 element that enhances p53-mediated transactivation when placed adjacent to the consensus p53 site. The GC3 element alone cannot act as a positive p53-responsive element. We also show that p53 activates transcription of the c-erbA-α gene through a DNA element that consists of the p53 quarter-sites and GC3 elements. Using gel mobility-shift assay, we identified a possible GC3 binding factor distinct from p53.

Original languageEnglish (US)
Pages (from-to)2059-2065
Number of pages7
JournalOncogene
Volume8
Issue number8
StatePublished - Aug 1993
Externally publishedYes

Fingerprint

Transcriptional Activation
erbA Genes
Binding Sites
DNA
Consensus Sequence
Electrophoretic Mobility Shift Assay
Gels

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Identification of a DNA element that can enhance p53-mediated transactivation. / Shiio, Yuzuru; Yamamoto, Tadashi; Yamaguchi, Nobuo.

In: Oncogene, Vol. 8, No. 8, 08.1993, p. 2059-2065.

Research output: Contribution to journalArticle

Shiio, Y, Yamamoto, T & Yamaguchi, N 1993, 'Identification of a DNA element that can enhance p53-mediated transactivation', Oncogene, vol. 8, no. 8, pp. 2059-2065.
Shiio, Yuzuru ; Yamamoto, Tadashi ; Yamaguchi, Nobuo. / Identification of a DNA element that can enhance p53-mediated transactivation. In: Oncogene. 1993 ; Vol. 8, No. 8. pp. 2059-2065.
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