Identification of a cell surface component of Swiss 3T3 cells associated with an inhibition of cell division

S. S. Lakshmanarao, W. E. Toole-Simms, H. K. Fattaey, R. J. Leach, T. C. Johnson

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4 Scopus citations


Polyclonal IgG, prepared to a purified bovine cell surface sialoglycopeptide (SGP) inhibitor of cell division, was used to identify an antigenically related molecule on the surface of Swiss 3T3 cells. SDS-PAGE and Western analyses showed that the anti-SGP antibody was monospecific and primarily recognized a 66-kDa protein of 3T3 cell membranes. Treatment of intact 3T3 cells or 3T3 cell membranes with either broad and phosphatidylinositol-specific phospholipase C enzymes suggested that the antigenic material most likely existed as an integral membrane molecule, or associated as a multimeric complex, and was not anchored at the cell surface by a phospholipid. The addition of anti-SGP IgG to 3T3 cell monolayer cultures was shown to promote cell division, suggesting a regulatory function for the membrane-associated molecule.

Original languageEnglish (US)
Pages (from-to)412-415
Number of pages4
JournalExperimental Cell Research
Issue number2
Publication statusPublished - Aug 1991


ASJC Scopus subject areas

  • Cell Biology

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