Identification of a cDNA encoding a retinoid X receptor homologue from Schistosoma mansoni: Evidence for a role in female-specific gene expression

Wendy J. Freebern, Ahmed Osman, Edward G. Niles, Linda Christen, Philip T Loverde

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Schistosoma mansoni, a multicellular eukaryotic blood fluke, is a major cause of morbidity worldwide in humans. The study of female parasite growth, development, and gene regulation is important because the eggs produced are responsible for the pathogenesis observed in schistosomiasis. p14, an eggshell precursor gene expressed only in sexually mature females in response to a male stimulus, is a model for female-specific gene regulation. The upstream region of the p14 gene shares sequences present in insect genes known to be regulated in a sex-, temporal-, and tissue-specific manner by members of the steroid receptor superfamily. Herein, we report the identification and characterization of a cDNA that encodes the S. mansoni (Sm) RXR homologue. Sequence analysis predicts and Western blot analysis confirms the synthesis of a 74-kDa protein, the largest member of the RXR family reported to date. We show by electrophoretic mobility shift assay analysis that SmRXR binds to cis-elements of the p14 gene including a direct repeat that follows the '3-4-5' rule of binding elements recognized by members of the steroid receptor superfamily. Furthermore, we demonstrate that SmRXR can act as a transcription activator in the yeast one-hybrid system. Through quantitative reverse transcriptase-polymerase chain reaction, we show that the SmRXR gene is constitutively expressed and thus must play multiple roles throughout the schistosome life cycle.

Original languageEnglish (US)
Pages (from-to)4577-4585
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number8
DOIs
StatePublished - Feb 19 1999
Externally publishedYes

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Retinoid X Receptors
Schistosoma mansoni
Gene expression
Complementary DNA
Genes
Gene Expression
Steroid Receptors
Insect Genes
Electrophoretic mobility
Egg Shell
Nucleic Acid Repetitive Sequences
Polymerase chain reaction
RNA-Directed DNA Polymerase
Transcription
Trematoda
Hybrid systems
Yeast
Schistosomiasis
Electrophoretic Mobility Shift Assay
Life cycle

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification of a cDNA encoding a retinoid X receptor homologue from Schistosoma mansoni : Evidence for a role in female-specific gene expression. / Freebern, Wendy J.; Osman, Ahmed; Niles, Edward G.; Christen, Linda; Loverde, Philip T.

In: Journal of Biological Chemistry, Vol. 274, No. 8, 19.02.1999, p. 4577-4585.

Research output: Contribution to journalArticle

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